caa a22 4500 477118771 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00250991 doi (NATIONALLICENCE)springer-10.1007/BF00250991 Leishmania donovani [Elektronische Daten] Metabolite mapping of promastigotes using proton nuclear magnetic resonance spectroscopy [U.K. Singha, Vinod Bhakuni, Vahab Ali, Raja Roy] Proton nuclear magnetic resonance spectroscopy was used for studying the intracellular metabolite profile of promastigotes of Leishmania donovani. The major intracellular metabolites observed in the promastigotes were acetate, alanine, succinate, glycine, α-glycerophosphorylcholine, acetoacetate, arginine and ethanol. A comparative study of the intracellular metabolite profile of promastigotes of different strains of L. donovani showed that, all the major intracellular metabolites were present in promastigotes of different strains. A quantitative estimation of metabolites showed a strain specific (Finger print) metabolite profile which can be used for strain/species identification/differentiation. Kluwer Academic Publishers, 1996 Leishmania donovani nationallicence promastigotes nationallicence metabolites nationallicence nuclear magnetic resonance (NMR) nationallicence perchloric acid extract (PCA) nationallicence strain differentiation nationallicence Singha U.K. Division of Membrane Biology, Central Drug Research Institute, Lucknow, India aut Bhakuni Vinod Division of Membrane Biology, Central Drug Research Institute, Lucknow, India aut Ali Vahab Division of Membrane Biology, Central Drug Research Institute, Lucknow, India aut Roy Raja Medicinal Chemistry (RSIC), Central Drug Research Institute, Lucknow, India aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 17-22 0300-8177 162:1<17 1996 162 11010 https://doi.org/10.1007/BF00250991 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00250991 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singha U.K. Division of Membrane Biology, Central Drug Research Institute, Lucknow, India aut NATIONALLICENCE 700 1- Bhakuni Vinod Division of Membrane Biology, Central Drug Research Institute, Lucknow, India aut NATIONALLICENCE 700 1- Ali Vahab Division of Membrane Biology, Central Drug Research Institute, Lucknow, India aut NATIONALLICENCE 700 1- Roy Raja Medicinal Chemistry (RSIC), Central Drug Research Institute, Lucknow, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 17-22 0300-8177 162:1<17 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer