caa a22 4500 47711878X CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00250995 doi (NATIONALLICENCE)springer-10.1007/BF00250995 Differential regulation of GRP78 and GLUT1 expression in 3T3-L1 adipocytes [Elektronische Daten] [Harvey Kitzman Jr., Robert McMahon, Ara Aslanian, Payal Fadia, Susan Frost] We tested the hypothesis that the constitutive glucose transporter (GLUT 1) in 3T3-L 1 adipocytes belongs to the family of glucose-regulated proteins which are transcriptionally regulated by glucose deprivation. Using cDNA probes for both GRP78 (BiP) and GLUT1, we show that the level of GRP78 mRNA increased by 15-fold within 24 h of glucose deprivation with little change in GLUT1 mRNA. The elevated GRP78 mRNA in turn led to a time-dependent increase in GRP78 protein. While glucose deprivation did not alter the expression of the normal glycoform of GLUT 1, a lower molecular weight glycoform accumulated with extended deprivation. Mannose and fructose, but not galactose, prevented the induction of GRP78 and accumulation of the abnormal GLUT1. Because GRP78 acts as a chaperone in other cell systems, we also sought evidence to support this activity in 3T3-L1 adipocytes. Using the technique of co-immunoprecipitation, we demonstrate that GRP78 bound several proteins unique to the glucose-deprived state. No deprivation-specific proteins could be detected in association with GLUT 1. These data lead us to conclude that GLUTl does not display characteristics of the glucose-regulated proteins, at least in 3T3-L1 adipocytes, a widely used model for differentiation, hormone action, and nutrient control. However, the mechanisms for activating traditional members of this family appear intact. Kluwer Academic Publishers, 1996 metabolism nationallicence glucose transporter nationallicence adipocytes nationallicence gene expression nationallicence Kitzman Jr. Harvey The Department of Biochemistry and Molecular Biology, University of Florida, Box 100245, JHMHC, 32610, Gainesville, FL, USA aut McMahon Robert Department of Food Science and Human Nutrition, University of Florida, Box 110370, 32611, Gainesville, FL, USA aut Aslanian Ara The Department of Biochemistry and Molecular Biology, University of Florida, Box 100245, JHMHC, 32610, Gainesville, FL, USA aut Fadia Payal The Department of Biochemistry and Molecular Biology, University of Florida, Box 100245, JHMHC, 32610, Gainesville, FL, USA aut Frost Susan The Department of Biochemistry and Molecular Biology, University of Florida, Box 100245, JHMHC, 32610, Gainesville, FL, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 51-58 0300-8177 162:1<51 1996 162 11010 https://doi.org/10.1007/BF00250995 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00250995 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kitzman Jr Harvey The Department of Biochemistry and Molecular Biology, University of Florida, Box 100245, JHMHC, 32610, Gainesville, FL, USA aut NATIONALLICENCE 700 1- McMahon Robert Department of Food Science and Human Nutrition, University of Florida, Box 110370, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Aslanian Ara The Department of Biochemistry and Molecular Biology, University of Florida, Box 100245, JHMHC, 32610, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Fadia Payal The Department of Biochemistry and Molecular Biology, University of Florida, Box 100245, JHMHC, 32610, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Frost Susan The Department of Biochemistry and Molecular Biology, University of Florida, Box 100245, JHMHC, 32610, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 51-58 0300-8177 162:1<51 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer