caa a22 4500 477118828 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00250997 doi (NATIONALLICENCE)springer-10.1007/BF00250997 Cyclic AMP regulates basement membrane heparan sulfate proteoglycan, perlecan, metabolism in rat glomerular epithelial cells [Elektronische Daten] [Cheol Ko, Basant Bhandari, J. Yee, W. Terhune, R. Maldonado, B. Kasinath] Perlecan, the basement membrane heparan sulfate proteoglycan (HSPG), has been fully cloned from mouse and human tissues. When a cRNA probe of murine perlecan cDNA was employed in RNase protection assay to test whether rat glomerular epithelial cells (GEC) constitutively express perlecan, several bands of hybridization were seen, suggesting that sequences between rat and murine perlecan may not be identical. Using primers based on published cDNA sequences of murine and human perlecan and polyA+ RNA of rat GEC, we synthesized a 497 by product (RPD-1) by RT PCR. The deduced aminoacid sequence showed an 85% and 88% homology with domain I of murine and human perlecan, respectively. The three putative sites containing the consensus sequence SGD for attachment of heparan sulfate chains were fully conserved in the rat perlecan as was a site (NFT) for attachment of N-linked oligosaccharide. RPD-I detected a > 9.5 kb transcript of perlecan in RNA of GEC, similar in size to that present in rat glomeruli. Employing a riboprobe synthesized from RPD-I in RNase protection assay we examined whether dbcAMP regulated perlecan expression in the GEC. At 1, 6, 24 and 48 h of incubation, l mM dbcAMP caused 43%, 32%, 47% and 40% reduction in mRNA abundance of perlecan, respectively. Immunoprecipitation showed a corresponding reduction of 61%, 70% and 65% in the synthesis of 35SO4 labeled basement membrane HSPG by the GEC following 12, 24 and 48 h of incubation with dbcAMP Following incubation for 1 and 24 h prostaglandins, PGE1 and PGE2 (1 uM), known activators of glomerular adenylate cyclase, reduced perlecan mRNA abundance to a similar extent as dbcAMP on northern analysis. Our results show that glomerular basement membrane HSPG synthesized by the GEC belongs to the perlecan family. Decrease of GEC perlecan gene expression and synthesis by cAMP and prostaglandins may be of relevance to proteinuric states characterized by activation of these mediators. Kluwer Academic Publishers, 1996 cAMP nationallicence prostaglandins nationallicence glomerular basement membrane nationallicence perlecan nationallicence glomerular epithelial cells nationallicence Ko Cheol Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut Bhandari Basant Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut Yee J. Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut Terhune W. Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut Maldonado R. Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut Kasinath B. Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 65-73 0300-8177 162:1<65 1996 162 11010 https://doi.org/10.1007/BF00250997 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00250997 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ko Cheol Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut NATIONALLICENCE 700 1- Bhandari Basant Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut NATIONALLICENCE 700 1- Yee J. Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut NATIONALLICENCE 700 1- Terhune W. Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut NATIONALLICENCE 700 1- Maldonado R. Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut NATIONALLICENCE 700 1- Kasinath B. Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans' Administration Hospital, San Antonio, Texas, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 65-73 0300-8177 162:1<65 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer