caa a22 4500 477118836 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00227533 doi (NATIONALLICENCE)springer-10.1007/BF00227533 Quantitation of glycerophosphorylcholine by flow injection analysis using immobilized enzymes [Elektronische Daten] [Alessandra Mancini, Francesca Del Rosso, Rita Roberti, Patrizio Caligiana, Alba Vecchini, Luciano Binaglia] A method for quantitating glycerophosphorylcholine by flow injection analysis is reported in the present paper. Glycerophosphorylcholine phosphodiesterase and choline oxidase, immobilized on controlled porosity glass beads, are packed in a small reactor inserted in a flow injection manifold. When samples containing glycerophosphorylcholine are injected, glycerophosphorylcholine is hydrolyzed into choline and sn-glycerol-3-phosphate. The free choline produced in this reaction is oxidized to betain and hydrogen peroxide. Hydrogen peroxide is detected amperometrically. Quantitation of glycerophosphorylcholine in samples containing choline and phosphorylcholine is obtained inserting ahead of the reactor a small column packed with a mixed bed ion exchange resin. The time needed for each determination does not exceed one minute. The present method, applied to quantitate glycerophosphorylcholine in samples of seminal plasma, gave results comparable with those obtained using the standard enzymatic- spectrophotometric procedure. An alternative procedure, making use of co-immobilized glycerophosphorylcholine phosphodiesterase and glycerol-3-phosphate oxidase for quantitating glycerophosphorylcholine, glycerophosphorylethanolamine and glycerophosphorylserine is also described. Kluwer Academic Publishers, 1996 flow injection analysis nationallicence glycerophosphorylcholine nationallicence glycerophosphorylethanolamine nationallicence glycerophosphorylserine nationallicence glycerophosphoric acid nationallicence glycerophosphorylcholine-phosphodiesterase nationallicence GPC : sn-glycerol-3-phosphorylcholine nationallicence GPE : sn-glycerol-3-phosphorylethanolamine nationallicence GPS : sn-glycerol-3-phosphorylserine nationallicence GPA : sn-glycerol-3-phosphoric acid nationallicence PDE : glycerophosphorylcholine-phosphodiesterase nationallicence GPA-Ox : glycerophosphate oxidase nationallicence Cho-Ox : choline oxidase nationallicence Mancini Alessandra Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut Del Rosso Francesca Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut Roberti Rita Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut Caligiana Patrizio Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut Vecchini Alba Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut Binaglia Luciano Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 83-87 0300-8177 162:2<83 1996 162 11010 https://doi.org/10.1007/BF00227533 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227533 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mancini Alessandra Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut NATIONALLICENCE 700 1- Del Rosso Francesca Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut NATIONALLICENCE 700 1- Roberti Rita Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut NATIONALLICENCE 700 1- Caligiana Patrizio Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut NATIONALLICENCE 700 1- Vecchini Alba Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut NATIONALLICENCE 700 1- Binaglia Luciano Institute of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Perugia, Via del Giochetto, 3-06100, Perugia, Italy aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 83-87 0300-8177 162:2<83 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer