caa a22 4500 477118844 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00227541 doi (NATIONALLICENCE)springer-10.1007/BF00227541 Calcium-binding protein regucalcin mRNA expression in the kidney cortex is suppressed by saline ingestion in rats [Elektronische Daten] [Nobuko Shinya, Hideyuki Kurota, Masayoshi Yamaguchi] The effect of adrenalectomy (ADX) or saline ingestion, which is a hypertensive factor, on the expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using rat liver regucalcin complementary DNA (0.9 kb of open-reading frame). Regucalcin mRNA was expressed in the kidney cortex but not the medulla. Rats were adrenalectomized, and 48 h later they were sacrificed. ADX caused a reduction of regucalcin mRNA levels in the kidney cortex, suggesting that adrenal glands participate in the regulation of the mRNA expression. This reduction was not restored by the subcutaneous administration of dexamethasone with an effective dose (1 mg/kg body weight), which can stimulate kidney regucalcin mRNA expression. Regucalcin mRNA levels in the kidney cortex of rats were markedly suppressed by the ingestion of saline for 7 days. The ADX-induced decrease of renal cortex regucalcin mRNA levels was not appreciably restored by saline ingestion. Moreover, regucalcin mRNA levels in the kidney cortex of spontaneous hypertensive rats (SHR) were clearly decreased as compared with that of control (Wistar-Kyoto) rats. Meanwhile, calcium content in the kidney cortex was not significantly decreased by ADX or saline ingestion. The present study suggests that the expression of regucalcin mRNA in the kidney cortex of rats is suppressed by saline administration. Kluwer Academic Publishers, 1996 regucalcin nationallicence calcium-binding protein nationallicence gene expression nationallicence saline ingestion nationallicence hypertensive rats nationallicence kidney cortex nationallicence Shinya Nobuko Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut Kurota Hideyuki Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut Yamaguchi Masayoshi Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 139-144 0300-8177 162:2<139 1996 162 11010 https://doi.org/10.1007/BF00227541 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227541 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shinya Nobuko Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut NATIONALLICENCE 700 1- Kurota Hideyuki Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut NATIONALLICENCE 700 1- Yamaguchi Masayoshi Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 139-144 0300-8177 162:2<139 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer