caa a22 4500 477118852 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00227542 doi (NATIONALLICENCE)springer-10.1007/BF00227542 Growth hormone stimulates the secretion of insulin-like growth factor binding protein-2 (IGFBP-2) by monolayer cultures of sheep costal growth plate chondrocytes [Elektronische Daten] [V. Borromeo, S. Bramani, A. Holder, C. Carter, C. Secchi, J. Beattie] Using monolayer cultures of costal chondrocytes established from four week old Clun Forest lambs, we have demonstrated that, under serum free conditions the cells release three IGFBPs (32, 29 and 21 kDa) into the medium. The most abundant of these—the 32 kDa BP-was shown to be IGFBP-2 by Western blotting. Furthermore we demonstrate that the levels of IGFBP 2 in conditioned medium are acutely increased (6, 12 and 24 h time points) following treatment of cells with bovine GH (1-100 ng/ml). In a parallel set of experiments, using ovine fibroblasts (derived from dermis) we show that IGFBPs of Mr 32, 29 and 21 kDa are also secreted by this cell type. However the relative abundance of these BPs differed from that seen in the chondrocyte cultures, with the 21 kDa species now the most abundant. In addition, prolonged exposure of autoradiographs indicated that fibroblasts secreted a higher Mr IGFBP (most probably ovine BP-3) that was not detected in any of our chondrocyte cultures. Most significant however was the demonstration that bGH did not dramatically affect the levels of IGFBPs in fibroblast cell cultures. We conclude that GH stimulates BP-2 production from chondrocytes and this is a cell-type specific effect in as much as it is not replicated in cultures of dermal fibroblasts. Kluwer Academic Publishers, 1996 ovine chondrocytes nationallicence growth hormone nationallicence IGFBPs nationallicence BCIP : 5-bromo-4-chloro-3-indolyl phosphate nationallicence CM : conditioned medium nationallicence DMEM : Dulbecco's Modified Eagles Medium nationallicence FCS : foetal calf serum nationallicence IGF : insulin-like growth factor nationallicence IGFBP : insulin-like growth factor binding protein nationallicence HBSS : Hanks' Balanced Salt Solution nationallicence GH : growth hormone nationallicence NBT : nitroblue tetrazolium nationallicence SFM : serum free medium nationallicence TBS : tris buffered saline nationallicence Borromeo V. Institute of Veterinary Physiology, University of Milan, Via Celoria 10, 20133, Milano, Italy aut Bramani S. Institute of Veterinary Physiology, University of Milan, Via Celoria 10, 20133, Milano, Italy aut Holder A. Babraham Institute, Babraham Hall, CB2 4AT, Babraham, Cambridge, England, UK aut Carter C. Babraham Institute, Babraham Hall, CB2 4AT, Babraham, Cambridge, England, UK aut Secchi C. Institute of Veterinary Physiology, University of Milan, Via Celoria 10, 20133, Milano, Italy aut Beattie J. Hannah Research Institute, K.46 SHL, Ayr, Scotland aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 145-151 0300-8177 162:2<145 1996 162 11010 https://doi.org/10.1007/BF00227542 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227542 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Borromeo V. Institute of Veterinary Physiology, University of Milan, Via Celoria 10, 20133, Milano, Italy aut NATIONALLICENCE 700 1- Bramani S. Institute of Veterinary Physiology, University of Milan, Via Celoria 10, 20133, Milano, Italy aut NATIONALLICENCE 700 1- Holder A. Babraham Institute, Babraham Hall, CB2 4AT, Babraham, Cambridge, England, UK aut NATIONALLICENCE 700 1- Carter C. Babraham Institute, Babraham Hall, CB2 4AT, Babraham, Cambridge, England, UK aut NATIONALLICENCE 700 1- Secchi C. Institute of Veterinary Physiology, University of Milan, Via Celoria 10, 20133, Milano, Italy aut NATIONALLICENCE 700 1- Beattie J. Hannah Research Institute, K.46 SHL, Ayr, Scotland aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 145-151 0300-8177 162:2<145 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer