caa a22 4500 477118879 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00227537 doi (NATIONALLICENCE)springer-10.1007/BF00227537 Regulation of ornithine decarboxylase and S-adenosylmethionine decarboxylase in a polyamine auxotrophic cell line [Elektronische Daten] [Fredrik Svensson, Lo Persson] In the present study we have examined the regulation of the polyamine biosynthetic pathway in a cell line deficient in ornithine decarboxylase (ODC) activity. These cells were unable to grow unless polyamines were provided in their growth medium. Seeding the cells in the absence of polyamines rapidly resulted in a cellular depletion of putrescine and spermidine. Although the cells were devoid of ODC activity they were demonstrated to express an inactive ODC which was feedback regulated by polyamines in a normal manner. Cells seeded in the absence of polyamines exhibited a marked increase in ODC synthesis rate which was not correlated with an equal change in the ODC mRNA level. The synthesis of S-adenosylmethionine decarboxylase (AdoMetDC) was also increased in the cells seeded in the absence of polyamines. However, this increase was essentially explained by a change in the amount of AdoMetDC mRNA. The addition of putrescine to the growth medium appeared to stimulate the conversion of AdoMetDC proenzyme into its two subunits, indicating a physiological role of putrescine in the regulation of AdoMetDC expression. Kluwer Academic Publishers, 1996 polyamines nationallicence ornithine decarboxylase nationallicence S-adenosylmethionine decarboxylase nationallicence Chinese hamster ovary cells nationallicence translational regulation nationallicence Svensson Fredrik Department of Physiology, University of Lund, S-223 62, Lund, Sweden aut Persson Lo Department of Physiology, University of Lund, S-223 62, Lund, Sweden aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 113-119 0300-8177 162:2<113 1996 162 11010 https://doi.org/10.1007/BF00227537 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227537 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Svensson Fredrik Department of Physiology, University of Lund, S-223 62, Lund, Sweden aut NATIONALLICENCE 700 1- Persson Lo Department of Physiology, University of Lund, S-223 62, Lund, Sweden aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 113-119 0300-8177 162:2<113 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer