caa a22 4500 477118917 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00227540 doi (NATIONALLICENCE)springer-10.1007/BF00227540 Enhancement of plasma membrane (Ca2+-Mg2+)-ATPase activity in regenerating rat liver: Involvement of endogenous activating protein regucalcin [Elektronische Daten] [Hiroko Takahasi, Masayoshi Yamaguchi] The alteration of (Ca2+-Mg2+)-ATPase activity in the plasma membranes of regenerating rat liver after a partial hepatectomy was investigated. Liver was surgically removed about two thirds of that of sham-operated rats. The reduced liver weight by partial hepatectomy was restored about 50% at 24 h after the surgery, and it was completely restored at 72 h. Regenerating liver significantly increased calcium content and plasma membrane (Ca2+-Mg2+)-ATPase activity between 12-48 h after hepatectomy. Those increases were maximum at 24 h after the surgery. The regenerating liver-induced increase in hepatic plasma membrane (Ca2+-Mg2+)-ATPase activity was completely abolished by the presence of anti-regucalcin IgG (1.0-4.0 μg/ml). The regenerating liver-induced increase in hepatic plasma membrane (Ca2+-Mg2+)-ATPase activity was clearly inhibited by N-ethylmaleimide (2.5 and 5.0 mM) addition into the enzyme reaction mixture. This NEM effect was also seen for the activatory effect with regucalcin (0.25 μM) addition on the enzyme activity in the plasma membranes from normal rat liver. The endogenous regucalcin may play a cell physiological role in the activation of the plasma membrane (Ca2+-Mg2+)-ATPase to maintain the intracellular calcium level in regenerating rat liver. Kluwer Academic Publishers, 1996 regucalcin nationallicence (Ca2+-Mg2+)-ATPase nationallicence calcium pump nationallicence plasma membrane nationallicence regenerating rat liver nationallicence cell proliferation nationallicence Takahasi Hiroko Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut Yamaguchi Masayoshi Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 133-138 0300-8177 162:2<133 1996 162 11010 https://doi.org/10.1007/BF00227540 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227540 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahasi Hiroko Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut NATIONALLICENCE 700 1- Yamaguchi Masayoshi Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 133-138 0300-8177 162:2<133 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer