caa a22 4500 477118933 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00227539 doi (NATIONALLICENCE)springer-10.1007/BF00227539 Suppression of β-oxidation restores pyruvate inhibition of pyruvate dehydrogenase kinase in starved rat heart [Elektronische Daten] [Tonia Carter, D. Dan Ramdath, Haldane Coore] Kinetic behaviour of rat heart pyruvate dehydrogenase kinase (PDHKα) was studied in the multi-enzyme complex (PDC) contained in two preparations: mitochondria (mPDC) and a high speed pellet of Triton-extracted tissue (hPDC). Two parameters were evaluated: Vaav, related to Vmax, and Fractional Pyruvate Inhibition (FPI). Starvation of rats for 48 h led to a rise in Vav and a fall in FPI. Injection into starved rats of agents which reduce β-oxidation of fatty acids restored, in succession, FPI and then Vav, of hPDC, to levels found in hPDC from fed animals. In vitro incubation at 30°C of hPDC from starved animals restored FPI, but not Vav, to ‘fed' values; both were restored during in vitro incubation of mPDC from starved animals within the same time frame as in the in vivo experiments. A sharp increase of FPI, but not Vav, of hPDC from both fed and starved rats was observed in later experiments. This could have been due to differential selection of the two genes for isoenzymes of PDHKa proposed by other workers. Kluwer Academic Publishers, 1996 pyruvate dehydrogenase kinase nationallicence rat heart nationallicence β-oxidation nationallicence pyruvate inhibition nationallicence Carter Tonia Biochemistry Department, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad, WI, USA aut Dan Ramdath D. Biochemistry Department, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad, WI, USA aut Coore Haldane Biochemistry Department, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad, WI, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 127-131 0300-8177 162:2<127 1996 162 11010 https://doi.org/10.1007/BF00227539 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227539 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carter Tonia Biochemistry Department, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad, WI, USA aut NATIONALLICENCE 700 1- Dan Ramdath D. Biochemistry Department, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad, WI, USA aut NATIONALLICENCE 700 1- Coore Haldane Biochemistry Department, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad, WI, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 162/2(1996-09-01), 127-131 0300-8177 162:2<127 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer