caa a22 4500 477118992 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00226064 doi (NATIONALLICENCE)springer-10.1007/BF00226064 Identification of conventional and novel endothelin receptors in sheep choroid plexus cells [Elektronische Daten] [Krassimira Angelova, Gregory Fralish, David Puett, Prema Narayan] Previous studies have demonstrated the presence of super-high affinity endothelin receptors with apparent Kd's on the order of pM in different brain tissues. This study was designed to characterize, in detail, the receptors present in SCP cells, a non-transformed sheep choroid plexus cell line. Competitive binding assays with receptor-selective ligands indicated the presence of at least three classes of binding sites: a conventional receptor of the ETA subtype with a Kd = 0.4 nM that mediates an increase in intracellular levels of inositol 1,4,5-trisphosphate (IP3) in response to ET-1 and two additional sites with much higher binding affinities. The latter two sites are not coupled to the common signal transduction pathways of IP3, cAMP and cGMP. Northern blot analysis confirmed the presence of only the ETA subtype mRNA in SCP cells. It remains to determined if the multiple binding sites are distinct gene products, multiple affinity states of a single receptor molecule or a result of cooperative association of one site with either the ligand or with other proteins. Kluwer Academic Publishers, 1996 endothelin receptors nationallicence choroid plexus cells nationallicence Angelova Krassimira Department of Biochemistry and Molecular Biology, University of Georgia, 30602, Athens, Georgia, USA aut Fralish Gregory Department of Biochemistry and Molecular Biology, University of Georgia, 30602, Athens, Georgia, USA aut Puett David Department of Biochemistry and Molecular Biology, University of Georgia, 30602, Athens, Georgia, USA aut Narayan Prema Department of Biochemistry and Molecular Biology, University of Georgia, 30602, Athens, Georgia, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/1(1996-06-01), 65-72 0300-8177 159:1<65 1996 159 11010 https://doi.org/10.1007/BF00226064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226064 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Angelova Krassimira Department of Biochemistry and Molecular Biology, University of Georgia, 30602, Athens, Georgia, USA aut NATIONALLICENCE 700 1- Fralish Gregory Department of Biochemistry and Molecular Biology, University of Georgia, 30602, Athens, Georgia, USA aut NATIONALLICENCE 700 1- Puett David Department of Biochemistry and Molecular Biology, University of Georgia, 30602, Athens, Georgia, USA aut NATIONALLICENCE 700 1- Narayan Prema Department of Biochemistry and Molecular Biology, University of Georgia, 30602, Athens, Georgia, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/1(1996-06-01), 65-72 0300-8177 159:1<65 1996 159 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer