caa a22 4500 47711900X CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00226057 doi (NATIONALLICENCE)springer-10.1007/BF00226057 The role of nitric oxide in the cardiac effects of hydrogen peroxide [Elektronische Daten] [Guro Wen, Tove Skjelbakken, Jarle Wage] Oxidative stress mediated by hydrogen peroxide (H2O2) increases coronary flow (CF) in Langendorff-perfused rat hearts. We investigated the possible role of nitric oxide (NO) in H2O2-induced vasolidation. A dose-response study was conducted to find a concentration of H2O2 which increased CF without influencing left ventricular developed (LVDP) or end-diastolic (LVEDP) pressures. 80 (n = 10),100 (n = 7), 120 (n = 7),140 (n = 7),160 (n = 7), and 180 (n = 10) μM H2O2 was infused for 10 min, followed by recovery for 50 min. 80 μM H2O2 increased CF to a maximum of 143 ± 4 (mean ± S.E.M) percent of initial value after 15 min observation (p < 0.001 compared to buffer only), with no effect on LVDP or LVEDP. Another series of hearts were perfused with N-nitro-L-Arginine methylester (L-NAME, 1 μM), methylene blue (MB, 50 μM), or haemoglobin (Hb, 10 μM), without (n = 7 in each) or with (n = 10 in each) 80 μM H2O2 for 10 min. L-NAME, MB, and Hb alone increased CF, but attenuated the H2O2-induced increase of CF. LVDP was depressed when L-NAME, MB, or Hb were given in conjunction with 80 μM H2O2. In summary, H2O2 concentration-dependently increased LVEDP and depressed LVDP. The H2O2-induced increase of CF was independent of concentration. Inhibition of NO synthesis, action, or soluble guanylate cyclase attenuated the H2O2-induced increase of CF, and depressed LVDP when given together with H2O2. H2O2 induces a NO-dependent vasodilation, and inhibition of NO is detrimental to left ventricular function after H2O2-mediated oxidative stress. Kluwer Academic Publishers, 1996 coronary circulation nationallicence heart nationallicence hydrogen peroxide nationallicence nitric oxide nationallicence oxidative stress nationallicence vasodilation nationallicence Wen Guro Department of Surgery, University Hospital of Tromso, Tromso, Norway aut Skjelbakken Tove Department of Surgery, University Hospital of Tromso, Tromso, Norway aut Wage Jarle Department of Surgery, University Hospital of Tromso, Tromso, Norway aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/1(1996-06-01), 7-14 0300-8177 159:1<7 1996 159 11010 https://doi.org/10.1007/BF00226057 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226057 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wen Guro Department of Surgery, University Hospital of Tromso, Tromso, Norway aut NATIONALLICENCE 700 1- Skjelbakken Tove Department of Surgery, University Hospital of Tromso, Tromso, Norway aut NATIONALLICENCE 700 1- Wage Jarle Department of Surgery, University Hospital of Tromso, Tromso, Norway aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/1(1996-06-01), 7-14 0300-8177 159:1<7 1996 159 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer