caa a22 4500 477119018 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00226063 doi (NATIONALLICENCE)springer-10.1007/BF00226063 Enzymes of carbohydrate metabolism in young and adult rats fed diets differing in fat and carbohydrate [Elektronische Daten] [Stephen Brooks, Brian Lampi] Glycogen content as well as glycolytic, gluconeogenic and fatty acid synthesis enzyme activities were monitored in young and adult male rats fed diets differing in fat content: 11 % (low), 22% (medium) and 42% (high) of total energy from fat. The results showed significant differences in the responses of young and adult rats to changes in dietary fat and carbohydrate. In young animals, increasing dietary fat decreased total liver glycogen phosphorylase (GP), pyruvate kinase (PK), glycerol 3-phosphate dehydrogenase, glucose 6-phosphate dehydrogenase, malic enzyme (ME), ATP-citrate lyase (ATP-CL) and fatty acid synthase (FAS). Increasing dietary fat also affected enzyme levels in other tissues: hexokinase (HK) and pyruvate dehydrogenase (PDH) activities decreased whereas skeletal muscle PK activity increased. The pattern of enzyme changes was similar in livers of fed adults with the exception that liver GP was not affected by dietary manipulations. Overnight food deprivation decreased liver glucokinase (GK), ME, ATP-CL, and FAS activities and increased liver phosphoenolpyruvate carboxykinase (PEPCK) and phosphofructokinase in both young and adult animals. In young animals, food deprivation also: (i) reduced liver GK and PK, (ii) increased kidney PEPCK, (iii) decreased muscle PEPCK and (iv) decreased kidney PDH. Food-deprived adults had increased skeletal muscle PEPCK and kidney glycogen synthetase as well as decreased kidney PEPCK muscle GP activity. These differences suggest that young animals are somewhat more responsive to changes in dietary manipulations. They also show that overnight food restriction causes a more profound metabolic re-organization in younger than in older animals. Kluwer Academic Publishers, 1996 metabolism nationallicence dietary fat nationallicence glycolysis nationallicence gluconeogenesis nationallicence fatty acid synthesis nationallicence Brooks Stephen Nutrition Research Division, Health Protection Branch, Health Canada, 3W Banting Research Centre, Tunney's Pasture, 2203C, K1A OL2, Ottawa, Ontario, Canada aut Lampi Brian Nutrition Research Division, Health Protection Branch, Health Canada, 3W Banting Research Centre, Tunney's Pasture, 2203C, K1A OL2, Ottawa, Ontario, Canada aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/1(1996-06-01), 55-63 0300-8177 159:1<55 1996 159 11010 https://doi.org/10.1007/BF00226063 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226063 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brooks Stephen Nutrition Research Division, Health Protection Branch, Health Canada, 3W Banting Research Centre, Tunney's Pasture, 2203C, K1A OL2, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Lampi Brian Nutrition Research Division, Health Protection Branch, Health Canada, 3W Banting Research Centre, Tunney's Pasture, 2203C, K1A OL2, Ottawa, Ontario, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/1(1996-06-01), 55-63 0300-8177 159:1<55 1996 159 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer