caa a22 4500 477119034 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00226060 doi (NATIONALLICENCE)springer-10.1007/BF00226060 Endothelin-1 induces tyrosine phosphorylation in human blood monocytes [Elektronische Daten] [Lindsey Chisholm, Devendra Agrawal, Trevor Pearson, John Edwards] Mediators including the neuropeptide endothelin-1 (ET-1), which are released in response to injury, modulate the expression of cell adhesion molecules on leukocytes and endothelial cells. The mechanisms underlying this process are not clear. In this study we investigated the effect of endothelin-1 on the expression of tyrosine phosphorylated proteins in human blood monocytes. Endothelin-1 caused an increase in tyrosine phosphorylated proteins in monocytes in a time-dependent and dose-dependent manner, the Mr 60, 80 and 110 kDa proteins being the most prominent. This effect was blocked by pre-incubating the monocytes with the selective tyrosine kinase inhibitors genistein or herbimycin A. Endothelin-1-induced upregulation of tyrosine phosphorylated proteins appears to be mediated by the ETAreceptor. Unlike our previously reported studies in endothelial cells, immunoprecipitation with anti-src or anti-JAK antibodies followed by immunoblotting with PY20 in human blood monocytes revealed that these proteins of Mr 60, 80 and 110 kDa were not related to src or JAK kinases. These findings suggest that ET-1 exerts its effect on monocytes by a pathway involving tyrosine kinases other than src or JAK kinases. Kluwer Academic Publishers, 1996 protein tyrosine kinase nationallicence monocytes nationallicence endothelin-1 nationallicence Chisholm Lindsey Department of Surgery, Creighton University School of Medicine, 68131, Omaha, Nebraska, USA aut Agrawal Devendra Department of Surgery, Creighton University School of Medicine, 68131, Omaha, Nebraska, USA aut Pearson Trevor Department of Surgery, Creighton University School of Medicine, 68131, Omaha, Nebraska, USA aut Edwards John Department of Surgery, Creighton University School of Medicine, 68131, Omaha, Nebraska, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/1(1996-06-01), 33-38 0300-8177 159:1<33 1996 159 11010 https://doi.org/10.1007/BF00226060 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226060 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chisholm Lindsey Department of Surgery, Creighton University School of Medicine, 68131, Omaha, Nebraska, USA aut NATIONALLICENCE 700 1- Agrawal Devendra Department of Surgery, Creighton University School of Medicine, 68131, Omaha, Nebraska, USA aut NATIONALLICENCE 700 1- Pearson Trevor Department of Surgery, Creighton University School of Medicine, 68131, Omaha, Nebraska, USA aut NATIONALLICENCE 700 1- Edwards John Department of Surgery, Creighton University School of Medicine, 68131, Omaha, Nebraska, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/1(1996-06-01), 33-38 0300-8177 159:1<33 1996 159 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer