caa a22 4500 477119050 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF00226058 doi (NATIONALLICENCE)springer-10.1007/BF00226058 Interspecies relationships among ADP-ribosylation factors (ARFs): Evidence of evolutionary pressure to maintain individual identities [Elektronische Daten] [S. Russ Price, Maria Nightingale, Mikako Tsuchiya, Joel Moss, Martha Vaughan] ADP-ribosylation factors (ARFs) are ∼20-kDa guanine nucleotide-binding proteins that are allosteric activators of the NAD:arginine ADP-ribosyltransferase activity of cholera toxin and appear to play a role in intracellular vesicular trafficking. Although the physiological roles of these proteins have not been defined, it has been presumed that each has a specific intracellular function. To obtain genetic evidence that each ARF is under evolutionary pressure to maintain its structure, and presumably function, rat ARF cDNA clones were isolated and their nucleotide and deduced amino acid sequences were compared to those of other mammalian ARFs. Deduced amino acid sequences for rat ARFs 1, 2, 3, 5 and 6 were identical to those of the known cognate human and bovine ARFs; rat ARF4 was 96% identical to human ARF4. Nucleotide sequences of both the untranslated as well as the coding regions were highly conserved. These results indicate that the ARF proteins are, as a family, extraordinarily well conserved across mammalian species. The unusually high degree of conservation of the untranslated regions is consistent with these regions having important regulatory roles and that individual ARFs contain structurally unique elements required for specific functions. Kluwer Academic Publishers, 1996 guanine nucleotide-binding proteins nationallicence evolution nationallicence phylogeny nationallicence structure-function nationallicence ARF : ADP-ribosylation factor nationallicence Gs : the stimulatory heterotrimeric guanine nucleotide-binding protein associated with adenylyl cyclase nationallicence SDS : sodium dodecyl sulfate nationallicence ORF : open reading frame nationallicence Russ Price S. Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, MD, USA aut Nightingale Maria Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, MD, USA aut Tsuchiya Mikako Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, MD, USA aut Moss Joel Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, MD, USA aut Vaughan Martha Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, MD, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/1(1996-06-01), 15-23 0300-8177 159:1<15 1996 159 11010 https://doi.org/10.1007/BF00226058 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00226058 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Russ Price S. Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, MD, USA aut NATIONALLICENCE 700 1- Nightingale Maria Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, MD, USA aut NATIONALLICENCE 700 1- Tsuchiya Mikako Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, MD, USA aut NATIONALLICENCE 700 1- Moss Joel Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, MD, USA aut NATIONALLICENCE 700 1- Vaughan Martha Pulmonary-Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 20892, MD, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/1(1996-06-01), 15-23 0300-8177 159:1<15 1996 159 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer