caa a22 4500 477119093 CHVBK 20180405111630.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00420913 doi (NATIONALLICENCE)springer-10.1007/BF00420913 Hepatic response to the oxidative stress induced by E. coli endotoxin: Glutathione as an index of the acute phase during the endotoxic shock [Elektronische Daten] [M. Portolés, Myriam Catalá, Adolfo Antón, Raffaella Pagani] Reactive oxygen species are important mediators of cellular damage during endotoxic shock. In order to investigate the hepatic response to the oxidative stress induced by endotoxin, hepatic and plasma glutathione (total, GSH and GSSG), GSSG/GSH ratio as well as Mn-superoxide dismutase and catalase activities were determined during the acute and recovery phases of reversible endotoxic shock in the rat. A significant increase in liver and plasma total glutathione content was observed 5 h after endotoxin treatment (acute phase), followed by a diminution of these parameters below control values at 48 h (recovery phase). The significant increases of GSSG levels and GSSG/GSH ratio are indicative of oxidative stress occurring during the acute phase. Liver Mn-SOD activity showed a similar time dependency as the GSSG/GSH ratio; however, a marked decrease in the liver catalase activity was observed during the process. These results indicate the participation of liver glutathione in the response to endotoxin and the possible use of plasma glutathione levels and GSSG/GSH ratio as indicators of the acute phase during the endotoxic process. (Mol Cell Biochem 159: 115-121, 1996) Kluwer Academic Publishers, 1996 endotoxin nationallicence free radicals nationallicence glutathione nationallicence superoxide dismutase nationallicence catalase nationallicence hepatocyte nationallicence Portolés M. Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Computense, 28040, Madrid, Spain aut Catalá Myriam Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Computense, 28040, Madrid, Spain aut Antón Adolfo Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Computense, 28040, Madrid, Spain aut Pagani Raffaella Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Computense, 28040, Madrid, Spain aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/2(1996-09-01), 115-121 0300-8177 159:2<115 1996 159 11010 https://doi.org/10.1007/BF00420913 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00420913 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Portolés M. Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Computense, 28040, Madrid, Spain aut NATIONALLICENCE 700 1- Catalá Myriam Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Computense, 28040, Madrid, Spain aut NATIONALLICENCE 700 1- Antón Adolfo Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Computense, 28040, Madrid, Spain aut NATIONALLICENCE 700 1- Pagani Raffaella Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Computense, 28040, Madrid, Spain aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/2(1996-09-01), 115-121 0300-8177 159:2<115 1996 159 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer