caa a22 4500 477119107 CHVBK 20180405111631.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00420914 doi (NATIONALLICENCE)springer-10.1007/BF00420914 Studies on L-threose as substrate for aldose reductase: A possible role in preventing protein glycation [Elektronische Daten] [P. Devamanoharan, S. Varma] L-threose is a product of ascorbate oxidation and degradation. By virtue of its free aldehyde group it can form Schiff-bases with tissue proteins, altering their normal function. In this study, we have examined the possibility of its detoxification to L-threitol by aldose reductase in the lens. The rat lens enzyme present in fresh homogenate as well as after 100 fold purification was found to utilize L-threose with a km of 7.1 × 10−4 M. The specificity of the reaction was affirmed by its inhibition with sorbinil and quercetin, the well known aldose reductase inhibitors. Further studies on the role of this enzyme in preventing toxicity due to degradation products of ascorbate are in progress. Kluwer Academic Publishers, 1996 aldose reductase nationallicence ascorbate nationallicence L-threose nationallicence glycation nationallicence cataracts nationallicence Devamanoharan P. Department of Ophthalmology, University of Maryland School of Medicine, 10 South Pine Street, 21201, Baltimore, MD, USA aut Varma S. Department of Ophthalmology, University of Maryland School of Medicine, 10 South Pine Street, 21201, Baltimore, MD, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/2(1996-09-01), 123-127 0300-8177 159:2<123 1996 159 11010 https://doi.org/10.1007/BF00420914 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00420914 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Devamanoharan P. Department of Ophthalmology, University of Maryland School of Medicine, 10 South Pine Street, 21201, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Varma S. Department of Ophthalmology, University of Maryland School of Medicine, 10 South Pine Street, 21201, Baltimore, MD, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/2(1996-09-01), 123-127 0300-8177 159:2<123 1996 159 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer