caa a22 4500 477119131 CHVBK 20180405111631.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00420919 doi (NATIONALLICENCE)springer-10.1007/BF00420919 Human recombinant interleukin-1β induces thromboxane A2 release in polymorphonuclear leukocytes, macrophages and platelets: Effect of IL-1 receptor antagonist [Elektronische Daten] [M. Reale, R. Barbacane, S. Frydas, G. Anogianakis, A. Trakatellis, D. Dimitriadou, D. Vacalis, F. Placido, P. De Fazio, E. Porreca, C. Di Febbo, P. Conti] Prostaglandins and thromboxanes (T×s) are produced by polymorphonuclears (PMNs) and macrophages (MØs) in response to various stimuli. PMNs were separated from other human blood cells and MOs were separated from rat peritoneal lavage. In this paper we show that human recombinant interleukin-1 (hrIL-1) can stimulate the release of thromboxane B2(T×B2) by PMNs and MØs. In addition, we have shown that aggregation of PMNs may occur when calcium ions (7 mM) and hrIL-1 (100 ng/ml) are added to the cell preparation, but not when Ca2+ alone, hrIL-1 alone, or first hrIL-1 then calcium are added to the cell preparation. The treatment of human platelets with hrIL-1 shows that after 15 min incubation T×B2 is released. In addition, we compared the aggregation of platelets caused by ADP with that caused by hrIL-1. Human recombinant IL-1 at a concentration of 100 ng/ml also causes little aggregation of platelets, in this case the aggregation is reversible. In conclusion, hrIL-1β stimulates T×B2 release in PMNs, MØs and platelets and this effect increases with addition of Ca2+ ions. The mixture of hrIL-1 and Ca2+ causes little aggregation of PMNs. In monocyte suspensions, pretreated with human recombinant IL-1 receptor antagonist (IL-Ira) 500 ng/ml for 10 min and then treated with LPS or hrIL-1β 10 μg/ml, the release of T×B2 was partially inhibited. IL-Ira may play a significant role in the control of IL-1 and LPS induction in the release of T×B2. Kluwer Academic Publishers, 1996 Reale M. Immunology Division, Institute of Experimental Medicine, Medical School, Greece aut Barbacane R. Immunology Division, Institute of Experimental Medicine, Medical School, Greece aut Frydas S. Department of Parasitology and Parasitic Diseases, Veterinary Faculty, Greece aut Anogianakis G. Department of Physiology, University of Thessaloniki, Greece aut Trakatellis A. Department ofBiochemistry, Faculty of Medicine, University of Thessaloniki, Greece aut Dimitriadou D. Department ofBiochemistry, Faculty of Medicine, University of Thessaloniki, Greece aut Vacalis D. Department ofBiochemistry, Faculty of Medicine, University of Thessaloniki, Greece aut Placido F. Immunology Division, Institute of Experimental Medicine, Medical School, Greece aut De Fazio P. Dental School, University "G. D Annunzio”, 66100, Chieti, Italy aut Porreca E. Institute of Medical Pathology, University "G. D Annunzio”, 66100, Chieti, Italy aut Di Febbo C. Institute of Medical Pathology, University "G. D Annunzio”, 66100, Chieti, Italy aut Conti P. Immunology Division, Institute of Experimental Medicine, Medical School, Greece aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/2(1996-09-01), 163-168 0300-8177 159:2<163 1996 159 11010 https://doi.org/10.1007/BF00420919 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00420919 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reale M. Immunology Division, Institute of Experimental Medicine, Medical School, Greece aut NATIONALLICENCE 700 1- Barbacane R. Immunology Division, Institute of Experimental Medicine, Medical School, Greece aut NATIONALLICENCE 700 1- Frydas S. Department of Parasitology and Parasitic Diseases, Veterinary Faculty, Greece aut NATIONALLICENCE 700 1- Anogianakis G. Department of Physiology, University of Thessaloniki, Greece aut NATIONALLICENCE 700 1- Trakatellis A. Department ofBiochemistry, Faculty of Medicine, University of Thessaloniki, Greece aut NATIONALLICENCE 700 1- Dimitriadou D. Department ofBiochemistry, Faculty of Medicine, University of Thessaloniki, Greece aut NATIONALLICENCE 700 1- Vacalis D. Department ofBiochemistry, Faculty of Medicine, University of Thessaloniki, Greece aut NATIONALLICENCE 700 1- Placido F. Immunology Division, Institute of Experimental Medicine, Medical School, Greece aut NATIONALLICENCE 700 1- De Fazio P. Dental School, University "G. D Annunzio”, 66100, Chieti, Italy aut NATIONALLICENCE 700 1- Porreca E. Institute of Medical Pathology, University "G. D Annunzio”, 66100, Chieti, Italy aut NATIONALLICENCE 700 1- Di Febbo C. Institute of Medical Pathology, University "G. D Annunzio”, 66100, Chieti, Italy aut NATIONALLICENCE 700 1- Conti P. Immunology Division, Institute of Experimental Medicine, Medical School, Greece aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 159/2(1996-09-01), 163-168 0300-8177 159:2<163 1996 159 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer