caa a22 4500 477119190 CHVBK 20180405111631.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00225836 doi (NATIONALLICENCE)springer-10.1007/BF00225836 Sex dependent immune responses by allogenic LDH isozymes [Elektronische Daten] [G. Gupta, G. Chaturvedi, A. Joshi] Regulatory effects on polyclonal activation of primed splenocytes have been studied following immunization through the intrarectal route with allogenic sperm specific lactate dehydrogenase (LDH-C4) and somatic LDH from kidney. Results indicate that LDH primed cell proliferation by mitogens is dependent on the nature of the isozyme and sex of donor cells. Compared to somatic LDH, LDH-C4 was immunosuppressive for T cell proliferation in vitro and the effect was more significant with female splenocytes as compared to male spleen cells. However, the suppressive effect of LDH-C4 on B cell function was identical in both males and females. In contrast to the somatic LDH which did not produce alloantibody in significant amount, LDH-C4 was highly immunogenic in production of humoral antibody in female mice. Alloantibody formation in dams was substantiated with a similar degree of immune regulation of B cell functions as shown by lipopolysaccharide stimulation. The role of LDH-C4 in protection of allogenic sperm in the female genital tract has been suggested. However, it is concluded that recipients of sperm constituents through the intrarectal route are at greater risk for immune suppression and bacterial/viral infection. Kluwer Academic Publishers, 1996 LDH-C4 nationallicence mitogenic stimulation nationallicence sperm specific antigen nationallicence sperm specific LDH nationallicence alloantibody nationallicence Gupta G. Department of Biophysics, Panjab University, Chandigarh and Institute of Microbial Technology, Sector-39, Chandigarh, India aut Chaturvedi G. Department of Biophysics, Panjab University, Chandigarh and Institute of Microbial Technology, Sector-39, Chandigarh, India aut Joshi A. Department of Biophysics, Panjab University, Chandigarh and Institute of Microbial Technology, Sector-39, Chandigarh, India aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 158/2(1996-05-01), 115-119 0300-8177 158:2<115 1996 158 11010 https://doi.org/10.1007/BF00225836 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00225836 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gupta G. Department of Biophysics, Panjab University, Chandigarh and Institute of Microbial Technology, Sector-39, Chandigarh, India aut NATIONALLICENCE 700 1- Chaturvedi G. Department of Biophysics, Panjab University, Chandigarh and Institute of Microbial Technology, Sector-39, Chandigarh, India aut NATIONALLICENCE 700 1- Joshi A. Department of Biophysics, Panjab University, Chandigarh and Institute of Microbial Technology, Sector-39, Chandigarh, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 158/2(1996-05-01), 115-119 0300-8177 158:2<115 1996 158 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer