caa a22 4500 477119220 CHVBK 20180405111631.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00225838 doi (NATIONALLICENCE)springer-10.1007/BF00225838 Interaction of a rat intestinal brush border membrane glycoprotein with type-1 fimbriae of Salmonella typhimurium [Elektronische Daten] [Sujata Ghosh, Amrisha Mittal, Harpreet Vohra, Nirmal Ganguly] Type-1 fimbriated Salmonella typhimurium was found to adhere to rat intestinal brush border membrane in a mannose sensitive manner. The maximum binding of the purified fimbriae observed with the rat illeal enterocytes was inhibited by 69.2% in presence of D-mannose. Brush border membrane from rat illeum was isolated, delipidified, solubilised and fractionated by affinity chromatography on type-1 fimbriae coupled Sepharose CL 4B column. Sodium dodecyl sulphate polyacrylamide gel electrophoresis of the material eluted from the column with D-mannose revealed a single band of molecular weight 60 kDa. The direct binding of this affinity eluted glycoprotein to the purified type-1 fimbriae was demonstrated by a modified Western blot experiment. Our findings suggest that the 60 kDa glycoprotein may serve as a receptor for the type-1 fimbriae in the rat intestinal brush border membrane and thereby may help in mediating bacterial adherence to the host epithelium. Kluwer Academic Publishers, 1996 Salmonella typhimurium nationallicence fimbriae nationallicence mannose nationallicence glycoprotein nationallicence receptor nationallicence Ghosh Sujata Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, India aut Mittal Amrisha Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, India aut Vohra Harpreet Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, India aut Ganguly Nirmal Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, India aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 158/2(1996-05-01), 125-131 0300-8177 158:2<125 1996 158 11010 https://doi.org/10.1007/BF00225838 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00225838 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ghosh Sujata Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, India aut NATIONALLICENCE 700 1- Mittal Amrisha Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, India aut NATIONALLICENCE 700 1- Vohra Harpreet Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, India aut NATIONALLICENCE 700 1- Ganguly Nirmal Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 158/2(1996-05-01), 125-131 0300-8177 158:2<125 1996 158 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer