caa a22 4500 477119247 CHVBK 20180405111631.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00225845 doi (NATIONALLICENCE)springer-10.1007/BF00225845 Rapid assay of HSF1 and HSF2 gene expression by RT-PCR [Elektronische Daten] [Xuan Ding, Robert Smallridge, Richard Galloway, Juliann Kiang] Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA. Kluwer Academic Publishers, 1996 reverse transcriptase polymerase chain reaction nationallicence gene nationallicence method nationallicence HSF1 nationallicence HSF2 nationallicence heat shock proteins nationallicence epithelia nationallicence Ding Xuan Department of Clinical Physiology, Division of Medicine, Walter Reed Army Institute of Research, 20307-5100, Washington, DC, USA aut Smallridge Robert Department of Clinical Physiology, Division of Medicine, Walter Reed Army Institute of Research, 20307-5100, Washington, DC, USA aut Galloway Richard Department of Clinical Physiology, Division of Medicine, Walter Reed Army Institute of Research, 20307-5100, Washington, DC, USA aut Kiang Juliann Department of Clinical Physiology, Division of Medicine, Walter Reed Army Institute of Research, 20307-5100, Washington, DC, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 158/2(1996-05-01), 189-192 0300-8177 158:2<189 1996 158 11010 https://doi.org/10.1007/BF00225845 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00225845 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ding Xuan Department of Clinical Physiology, Division of Medicine, Walter Reed Army Institute of Research, 20307-5100, Washington, DC, USA aut NATIONALLICENCE 700 1- Smallridge Robert Department of Clinical Physiology, Division of Medicine, Walter Reed Army Institute of Research, 20307-5100, Washington, DC, USA aut NATIONALLICENCE 700 1- Galloway Richard Department of Clinical Physiology, Division of Medicine, Walter Reed Army Institute of Research, 20307-5100, Washington, DC, USA aut NATIONALLICENCE 700 1- Kiang Juliann Department of Clinical Physiology, Division of Medicine, Walter Reed Army Institute of Research, 20307-5100, Washington, DC, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 158/2(1996-05-01), 189-192 0300-8177 158:2<189 1996 158 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer