caa a22 4500 477119298 CHVBK 20180405111631.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00225834 doi (NATIONALLICENCE)springer-10.1007/BF00225834 Atrial natriuretic peptide inhibits the phosphoinositide hydrolysis in murine Leydig tumor cells [Elektronische Daten] [Madan Khurana, Kailash Pandey] The ability of ANP to inhibit the hydrolysis of phosphoinositides was examined in [3H] myoinositol-labeled intact murine Leydig tumor (MA-10) cells. Arginine vasopressin (AVP) stimulated the formation of inositol monophosphate (IP1), inositol bisphosphate (IP2), and inositol trisphosphate (IP3) both in a time- and dose- dependent manner in MA-10 cells. ANP inhibited the AVP-induced formation of IP1, IP2, and IP3 in these cells. The inhibitory effect of ANP on the AVP-stimulated formation of IP1, IP2, and IP3 accounted for 30%, 38% and 42%, respectively, which was observed at the varying concentrations of AVP. ANP caused a dose-dependent attenuation in AVP-stimulated production of IP1, IP2 and IP3 with maximum inhibition at 100 nM concentration of ANP. The production of inositol phosphates was inhibited in the presence of 8- bromo cGMP in a dose-dependent manner, whereas dibutyryl-cAMP had no effect on the generation of these metabolites. The LY 83583, an inhibitor of guanylyl cyclase and cGMP production, abolished the inhibitory effect of ANP on the AVP-stimulated production of inositol phosphates. Furthermore, 10 μM LY 83583 also inhibited the ANP-stimulated guanylyl cyclase activity and the intracellular accumulation of cGMP by more than 65-70%. The inhibition of eGMP-dependent protein kinase by H-8, significantly restored the levels of AVP-stimulated inositol phosphates in the presence of either ANP or exogenous 8-bromo cGMP. The results of this study suggest that ANP exerts an inhibitory effect on the production of inositol phosphates in murine Leydig tumor (MA-10) cells by mechanisms involving cGMP and cGMP-dependent protein kinase. Kluwer Academic Publishers, 1996 atrial natriuretic peptide nationallicence arginine vasopressin nationallicence inositol phosphates nationallicence guanosine 3′, 5′ cyclic monophosphate nationallicence leydig tumor cells nationallicence Khurana Madan Department of Biochemistry & Molecular Biology, Medical College of Georgia, School of Medicine, 30912, Augusta, Georgia, USA aut Pandey Kailash Department of Biochemistry & Molecular Biology, Medical College of Georgia, School of Medicine, 30912, Augusta, Georgia, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 158/2(1996-05-01), 97-105 0300-8177 158:2<97 1996 158 11010 https://doi.org/10.1007/BF00225834 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00225834 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khurana Madan Department of Biochemistry & Molecular Biology, Medical College of Georgia, School of Medicine, 30912, Augusta, Georgia, USA aut NATIONALLICENCE 700 1- Pandey Kailash Department of Biochemistry & Molecular Biology, Medical College of Georgia, School of Medicine, 30912, Augusta, Georgia, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 158/2(1996-05-01), 97-105 0300-8177 158:2<97 1996 158 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer