caa a22 4500 47711931X CHVBK 20180405111631.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00225843 doi (NATIONALLICENCE)springer-10.1007/BF00225843 Zinc compounds inhibit osteoclast-like cell formation at the earlier stage of rat marrow culture but not osteoclast function [Elektronische Daten] [Masayoshi Yamaguchi, Seiko Kishi] The effect of zinc compounds on osteoclast-like cell formation in rat marrow culture in vitro was investigated. The bone marrow cells were cultured for 7 days in α-minimal essential medium containing a well-known bone resorbing hormone (1, 25-dihydroxyvitamin D3 and parathyroid hormone [1-34]). Osteoclast-like cell formation was estimated by staining for tartrateresistant acid phosphatase (TRACP), a marker enzyme of osteoclasts. The presence of 1, 25-dihydroxyvitamin D3 (10−8 M) or parathyroid hormone (PTH; 10−8 M) induced a remarkable increase in osteoclast-like multinucleated cells (MNC). These increases were clearly inhibited by the presence of zinc sulfate or zinc-chelating dipeptide (β-alanyl-L-histidinato zinc; AHZ) in the concentration range of 10−7 to 10−5 M. The inhibitory effect was seen at the earlier stage of osteoclast-like MNC formation. However, zinc compounds (10−6 M) did not have an effect on PTH (10−8 M)-induced osteoclast-like cell formation in the presence of EGTA (5 × 10−4 M), dibucaine (10−5 M) or staurosporine (10−9 M). Moreover, when osteoclasts isolated from rat femoraldiaphyseal tissues were cultured for 24 h in the presence of zinc compounds (10−7 to 10−5 M), the compounds did not have an effect on cell numbers or lysosomal enzymes activity (acid phosphatase and β-glucuronidase) in the cells. The present study clearly demonstrates that zinc compounds inhibit osteoclast-like cell formation at the earlier stage with differentiation of marrow cells. Kluwer Academic Publishers, 1996 zinc nationallicence β-alanyl-L-histidinato zinc nationallicence osteoclast formation nationallicence bone marrow cells nationallicence bone metabolism nationallicence Yamaguchi Masayoshi Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City 422, Japan aut Kishi Seiko Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City 422, Japan aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 158/2(1996-05-01), 171-177 0300-8177 158:2<171 1996 158 11010 https://doi.org/10.1007/BF00225843 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00225843 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi Masayoshi Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City 422, Japan aut NATIONALLICENCE 700 1- Kishi Seiko Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City 422, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 158/2(1996-05-01), 171-177 0300-8177 158:2<171 1996 158 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer