caa a22 4500 477119344 CHVBK 20180405111631.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00240030 doi (NATIONALLICENCE)springer-10.1007/BF00240030 Influence of glycosylation inhibitors on dihydropyridine binding to cardiac cells [Elektronische Daten] [Ursula Henning, Gerd Wallukat, Martin Holtzhauer] In primary cultures of neonatal rat heart cells we found a linear correlation between the number of L-type calcium channel-specific dihydropyridine (DHP) binding sites and spontaneous beating frequency (v). Formation of glycoproteins in tissue culture was suppressed by different inhibitors of N-glycosylation. This inhibition alters to a different extent the binding of the DHP ligand (+)-[methyl-3H]PN 200-110 and v. The most severe but reversible effect occurs at 6 μg/ml tunicamycin (Bmax ≈ 45% and v ≈ 6%, resp., of control), a slight increase in Bmax at 0.1-0.5 mM castanospermine and 0.05-2.5 mM deoxymannojirimycin. The other inhibitors gave no significant alterations of Bmax. Kluwer Academic Publishers, 1996 calcium channel nationallicence glycosylation inhibitor nationallicence cultured cardiac cells nationallicence dihydropyridine receptors nationallicence glycoproteins nationallicence Henning Ursula Department of Molecular Cardiology, Max Delbrück Center for Molecular Medicine, R.-Rössle-Str 10, D-13122, Berlin, Germany aut Wallukat Gerd Institute of Biochemistry and Molecular Physiology, University of Potsdam, Germany aut Holtzhauer Martin Department of Molecular Cardiology, Max Delbrück Center for Molecular Medicine, R.-Rössle-Str 10, D-13122, Berlin, Germany aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 47-52 0300-8177 160-161:1<47 1996 160-161 11010 https://doi.org/10.1007/BF00240030 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00240030 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Henning Ursula Department of Molecular Cardiology, Max Delbrück Center for Molecular Medicine, R.-Rössle-Str 10, D-13122, Berlin, Germany aut NATIONALLICENCE 700 1- Wallukat Gerd Institute of Biochemistry and Molecular Physiology, University of Potsdam, Germany aut NATIONALLICENCE 700 1- Holtzhauer Martin Department of Molecular Cardiology, Max Delbrück Center for Molecular Medicine, R.-Rössle-Str 10, D-13122, Berlin, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 47-52 0300-8177 160-161:1<47 1996 160-161 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer