caa a22 4500 477119379 CHVBK 20180405111631.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00240029 doi (NATIONALLICENCE)springer-10.1007/BF00240029 Primary cultures of cardiac muscle cells as models for investigation of protein glycosylation [Elektronische Daten] [Ursula Henning, Wolf-Peter Wolf, Martin Holtzhauer] Primary cardiac cell cultures of newborn rats containing approximately 50% (by cell number) spontaneously contracting cardiomyocytes were used to study the role of protein N-glycosylation for the binding of dihydropyridine (DHP) to the voltage-dependent L-type calcium channel. This binding is not influenced by the accompanying non-muscle cells. Exposure of the cells up to 6 μg/ml of the N-glycosylation inhibitor tunicamycin for a 44 h period resulted in a decrease of the specific DHP binding sites (Bmax) to 46.0 ± 17.2% of the untreated control. Similar effects were observed after enzymatic deglycosylation using N-glycosidase F (PNGase F). The results suggest that a posttranslational modification of parts of the cardiac L-type Ca++ channel by N-glycosylation is an important determinant for the binding of Ca++ antagonists of the DHP-type to the α1 subunit which itself is not glycosylated. The results suggest a participation of N glycosylation in the assembling of the subunits to the functional channel and/or its turnover. However, a possible effect of tunicamycin on the expression of the Ca channel as an alternative mechanism cannot be excluded. Kluwer Academic Publishers, 1996 calcium channel nationallicence glycosylation nationallicence glycoprotein nationallicence cardiac muscle cells nationallicence primary culture nationallicence Henning Ursula Institute of Biochemistry and Molecular Physiology, University of Potsdam, c/o Max Delbrück Center, Berlin, Germany aut Wolf Wolf-Peter Department of Molecular Cardiology, Max Delbrück Center, Robert-Rössle-Str. 10, D-13122, Berlin, Germany aut Holtzhauer Martin Institute of Biochemistry and Molecular Physiology, University of Potsdam, c/o Max Delbrück Center, Berlin, Germany aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 41-46 0300-8177 160-161:1<41 1996 160-161 11010 https://doi.org/10.1007/BF00240029 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00240029 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Henning Ursula Institute of Biochemistry and Molecular Physiology, University of Potsdam, c/o Max Delbrück Center, Berlin, Germany aut NATIONALLICENCE 700 1- Wolf Wolf-Peter Department of Molecular Cardiology, Max Delbrück Center, Robert-Rössle-Str. 10, D-13122, Berlin, Germany aut NATIONALLICENCE 700 1- Holtzhauer Martin Institute of Biochemistry and Molecular Physiology, University of Potsdam, c/o Max Delbrück Center, Berlin, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 41-46 0300-8177 160-161:1<41 1996 160-161 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer