caa a22 4500 477119417 CHVBK 20180405111632.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00240058 doi (NATIONALLICENCE)springer-10.1007/BF00240058 Donors of nitric oxide mimic effects of ischaemic preconditioning on reperfusion induced arrhythmias in isolated rat heart [Elektronische Daten] [Maria Bilińska, Michał Mączewski, Andrzej Beręsewicz] NO has been implicated in the mechanism of ischaemic preconditioning. To verify this hypothesis further we have attempted to reproduce effects of ischaemic preconditioning by nitric oxide donors administration prior to the ischaemia. The effect of glyceryl trinitrate (GTN) and 3-morpholino-sydnonimine-hydrochloride (SIN- 1), NO donors, on reperfusion induced ventricular tachycardia (VT) and ventricular fibrillation (VF) in Langendorff perfused rat hearts subjected to 10 min regional ischaemia followed by 10 min reperfusion were examined. Results: GTN, 500 μM and SIN-1, 10 μM, administered for 5 min and washed for another 5 min prior to ischaemia (to mimic ischaemic preconditioning), almost completely abolished reperfusion induced VF. GTN and SIN-1, administered at the time of reperfusion, increased the incidence of sustained VF and the duration of VT and VF. When given 5 min before the ischaemia and throughout the ischaemia and the reperfusion, SIN-1 abolished VF. Adenosine, 10 μM, applied according to the above three protocols, did not affect reperfusion arrhythmias, although adenosine induced changes in coronary flow and post-ischaemic reflow were similar to those produced by the NO donors. In conclusions: (1) NO is able to mimic the effect of ischaemic preconditioning on reperfusion arrhythmias in rat heart, supporting the view that NO may be one of the endogenous substances triggering ischaemic preconditioning; (2) In crystalloid-perfused heart, NO may be deleterious when its administration is restricted to the reperfusion period. Kluwer Academic Publishers, 1996 nitric oxide nationallicence preconditioning nationallicence reperfusion arrhythmias nationallicence isolated rat heart nationallicence SIN-1 nationallicence glyceryl trinitrate nationallicence adenosine nationallicence nitric oxide toxicity nationallicence Bilińska Maria Department of Clinical Physiology, Medical Centre of Postgraduate Education, Marymoncka 99, 01-813, Warsaw, Poland aut Mączewski Michał Department of Clinical Physiology, Medical Centre of Postgraduate Education, Marymoncka 99, 01-813, Warsaw, Poland aut Beręsewicz Andrzej Department of Clinical Physiology, Medical Centre of Postgraduate Education, Marymoncka 99, 01-813, Warsaw, Poland aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 265-271 0300-8177 160-161:1<265 1996 160-161 11010 https://doi.org/10.1007/BF00240058 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00240058 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bilińska Maria Department of Clinical Physiology, Medical Centre of Postgraduate Education, Marymoncka 99, 01-813, Warsaw, Poland aut NATIONALLICENCE 700 1- Mączewski Michał Department of Clinical Physiology, Medical Centre of Postgraduate Education, Marymoncka 99, 01-813, Warsaw, Poland aut NATIONALLICENCE 700 1- Beręsewicz Andrzej Department of Clinical Physiology, Medical Centre of Postgraduate Education, Marymoncka 99, 01-813, Warsaw, Poland aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 265-271 0300-8177 160-161:1<265 1996 160-161 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer