caa a22 4500 477119433 CHVBK 20180405111632.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00240040 doi (NATIONALLICENCE)springer-10.1007/BF00240040 Functional antagonism by a monoclonal antibody to digoxin in a test system of cultured rat heart myocytes [Elektronische Daten] [G. Wallukat, H. Simon, W. Müller, I. Wolf] A monoclonal antibody with a high affinity for digitoxin (KA = 0.50 nM) and digoxin (KA = 0.55 nM) was produced by somatic cell fusion. This antibody, designated 2A3(47), displayed little cross reactivity with other glycosides. In cultured rat heart myocytes, 2A3(47), antagonized the positive chronotropic effect exerted by digitoxin but did not alter that of ouabain. Our results suggest that this monoclonal antibody may prove to be useful in treating digoxin and digitoxin intoxication. Kluwer Academic Publishers, 1996 glycosides nationallicence monoclonal antibodies nationallicence cardiac myocytes nationallicence cell culture nationallicence digitoxin intoxication nationallicence Wallukat G. Max Delbrück Centre for Molecular Medicine, Robert-Rössle-Str. 10, 13125, Berlin-Buch, Germany aut Simon H. Institute of Clinical Immunology, Friedrich-Schiller Universität Jena, Am Johannesfriedhof 3, 07740, Jena, Germany aut Müller W. Institute of Clinical Immunology, Friedrich-Schiller Universität Jena, Am Johannesfriedhof 3, 07740, Jena, Germany aut Wolf I. Institute of Clinical Immunology, Friedrich-Schiller Universität Jena, Am Johannesfriedhof 3, 07740, Jena, Germany aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 117-120 0300-8177 160-161:1<117 1996 160-161 11010 https://doi.org/10.1007/BF00240040 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00240040 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wallukat G. Max Delbrück Centre for Molecular Medicine, Robert-Rössle-Str. 10, 13125, Berlin-Buch, Germany aut NATIONALLICENCE 700 1- Simon H. Institute of Clinical Immunology, Friedrich-Schiller Universität Jena, Am Johannesfriedhof 3, 07740, Jena, Germany aut NATIONALLICENCE 700 1- Müller W. Institute of Clinical Immunology, Friedrich-Schiller Universität Jena, Am Johannesfriedhof 3, 07740, Jena, Germany aut NATIONALLICENCE 700 1- Wolf I. Institute of Clinical Immunology, Friedrich-Schiller Universität Jena, Am Johannesfriedhof 3, 07740, Jena, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 117-120 0300-8177 160-161:1<117 1996 160-161 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer