caa a22 4500 477119506 CHVBK 20180405111632.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00240053 doi (NATIONALLICENCE)springer-10.1007/BF00240053 Delayed cardioprotection is associated with the sub-cellular relocalisation of ventricular protein kinase Cɛ, but not p42/44MAPK [Elektronische Daten] [Susan Wilson, Wu Song, Kaszala Karoly, Tanya Ravingerova, Agnes Vegh, Juluisby Papp, Shogo Tomisawa, James Parratt, Nigel Pyne] Both noradrenaline administration to rats and rapid cardiac pacing in dogs induces delayed protection of the heart against ischaemia-induced ventricular arrhythmias. In an attempt to establish molecular mechanisms underlying the delayed cardioprotection, we have examined the potential role of two kinases, PKCɛ and p42/44MAPK. These protein kinases are expressed in the ventricles of the heart and are characterised by their ability to regulate ion-flux and gene transcription. In the rat p42MAPK is predominantly localised in the high-speed supernatant fraction of the ventricle homogenate, whereas p44MAPK is enriched in the nuclear low speed pellet. A small proportion of the p42MAPK is activated even in hearts from control animals. However, neither kinase is relocalised or activated by noradrenaline administration and this provides preliminary evidence that p42/44MAPK may not play a significant role in delayed protection in this species. In contrast, noradrenaline does induce the translocation of PKCɛ to cell membranes, a response that is sustained for up to 4 h. However, PKCɛ is down-regulated from the cytoplasm after 24 h post noradrenaline treatment. PKCE is also translocated to the membrane in dogs that have been classically pre-conditioned and cardiac paced. In the latter case, translocation of PKCɛ from the cytoplasm to the cell membrane is evident 24 h after pacing. These results indicate that the release of endogenous mediators may either inhibit down-regulation or elicit an increase in PKCɛ mRNA expression. Therefore, in dog heart the subcellular relocalisation of PKCɛ persists into the ‘second window' and may play a central role in the molecular mechanism governing delayed cardioprotection. It is important in the future to identify either the gene products that are induced or the target protein(s) that are phosphorylated by PKCɛ. Kluwer Academic Publishers, 1996 classical preconditioning nationallicence noradrenaline nationallicence P42/44MAPK nationallicence protein kinase Cɛ nationallicence nuclear transcription nationallicence heat shock proteins nationallicence immediate early genes nationallicence Wilson Susan Department of Physiology and Pharmacology, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, Scotland aut Song Wu Department of Physiology and Pharmacology, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, Scotland aut Karoly Kaszala Albert Szent-Gyorgi Medical School, Doutef 12, Szeged, Hungary aut Ravingerova Tanya Department of Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic aut Vegh Agnes Albert Szent-Gyorgi Medical School, Doutef 12, Szeged, Hungary aut Papp Juluisby Albert Szent-Gyorgi Medical School, Doutef 12, Szeged, Hungary aut Tomisawa Shogo Chugai Pharmac Company LTD, 2-1-9 Kyobashi, Chuo-Ku, Tokyo, Japan aut Parratt James Department of Physiology and Pharmacology, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, Scotland aut Pyne Nigel Department of Physiology and Pharmacology, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, Scotland aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 225-230 0300-8177 160-161:1<225 1996 160-161 11010 https://doi.org/10.1007/BF00240053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00240053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wilson Susan Department of Physiology and Pharmacology, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, Scotland aut NATIONALLICENCE 700 1- Song Wu Department of Physiology and Pharmacology, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, Scotland aut NATIONALLICENCE 700 1- Karoly Kaszala Albert Szent-Gyorgi Medical School, Doutef 12, Szeged, Hungary aut NATIONALLICENCE 700 1- Ravingerova Tanya Department of Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Vegh Agnes Albert Szent-Gyorgi Medical School, Doutef 12, Szeged, Hungary aut NATIONALLICENCE 700 1- Papp Juluisby Albert Szent-Gyorgi Medical School, Doutef 12, Szeged, Hungary aut NATIONALLICENCE 700 1- Tomisawa Shogo Chugai Pharmac Company LTD, 2-1-9 Kyobashi, Chuo-Ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Parratt James Department of Physiology and Pharmacology, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, Scotland aut NATIONALLICENCE 700 1- Pyne Nigel Department of Physiology and Pharmacology, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, Scotland aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 225-230 0300-8177 160-161:1<225 1996 160-161 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer