caa a22 4500 477119514 CHVBK 20180405111632.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00240034 doi (NATIONALLICENCE)springer-10.1007/BF00240034 Involvement of nitric oxide synthase and protein kinase C activation on chagasic antibodies action upon cardiac contractility [Elektronische Daten] [Leonor Sterin-Borda, Graciela Cremaschi, Ana Genaro, Agustina Echagüe, Juan Goin, Enri Borda] We have already demonstrated the presence of antibodies in the sera of chagasic patients with the ability to interact with neurotransmitter receptors triggering several intracellular pathways of transduction signals. Here we show that, chagasic IgG induced protein kinase C (PKC) translocation to rat cardiac membranes and this effect was inhibited by muscarinic cholinergic blockers atropine and AF-DX 116 pointing to the participation of M2 receptors in this effect. It was also able to stimulate nitric oxide synthase (NOS) activity and this action was blunted by phospholipase C (PLC) and PKC inhibitors indicating that the production of nitric oxide (NO) would be the consequence of the cascade of enzymatic pathways triggered by mAChR activation. PKC and NOS activities were involved in chagasic IgG negative inotropic actions on rat isolated myocardium as its effects were blunted by staurosporine and L-N-monomethyl arginine. Furthermore, low concentrations of chagasic IgG inhibited the cardiac mechanical action of carbachol in a non-competitive manner. These data suggested that PKC activation in myocardium by chagasic IgG would be involved in its physiological actions by modulating NOS activity. The participation of PKC-mediated phosphorylation of mAChR leading to receptor desensitization as one of the causes of dysautonomia is also discussed. Kluwer Academic Publishers, 1996 atria contractility nationallicence protein kinase C nationallicence nitric oxide nationallicence muscarinic cholinergic receptors nationallicence chagasic IgG nationallicence Sterin-Borda Leonor Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut Cremaschi Graciela Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut Genaro Ana Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut Echagüe Agustina Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut Goin Juan Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut Borda Enri Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 75-82 0300-8177 160-161:1<75 1996 160-161 11010 https://doi.org/10.1007/BF00240034 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00240034 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sterin-Borda Leonor Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Cremaschi Graciela Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Genaro Ana Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Echagüe Agustina Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Goin Juan Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Borda Enri Cefybo-Conicet and Department of Pharmacology School of Medicine and Dentistry, University of Buenos Aires, Serrano 669, 1414, Buenos Aires, Argentina aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 75-82 0300-8177 160-161:1<75 1996 160-161 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer