caa a22 4500 47711959X CHVBK 20180405111632.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00240049 doi (NATIONALLICENCE)springer-10.1007/BF00240049 Relation between energy metabolism, glycolysis, noradrenaline release and duration of ischemia [Elektronische Daten] [Anna Cargnoni, Claudio Ceconi, Salvatore Curello, Massimo Benigno, Jan de Jong, Roberto Ferrari] We studied the effect of 12-36 min of global ischemia followed by 36 min of reperfusion in Langendorff perfused rabbit hearts (n = 26). Metabolism was determined in terms of peak and total release of purines (adenosine, inosine, hypoxanthine), lactate and noradrenaline during reperfusion; and myocardial content of nucleotides (ATP, ADP, AMP), glycogen and noradrenaline at the end of reperfusion. An inverse relationship (r = −0.79) existed between duration of ischemia and developed pressure post-ischemia. Early during reperfusion, after 12 min of ischemia, the purine concentration (peak release) increased 100x (p < 0.01), that of lactate and noradrenaline lOx (p < 0.05) . Total purine release rose with progression of the ischemic period (30x after 36 min of ischemia; p < 0.01), concomitant with a reduction in nucleotide content. Lactate release was independent from the duration of ischemia, although glycogen had declined by 30% (p < 0.01) after 36 min of ischemia. The acid insoluble glycogen fraction, which presumably contains proglycogen, increased substantially during short-term ischemia. Peak noradrenaline increased 100x and 200x (p < 0.05) after 24 and 36 min of ischemia, respectively. Total noradrenaline release due to various periods of ischemia mirrored its peak release. Function recovery was inversely related to total purine and noradrenaline efflux (both r =−0.81); it correlated with tissue nucleotide content (r = 0.84). In conclusion, larger amounts of noradrenaline are released only after a substantial drop in myocardial ATP. During severe ischemia ATP consumption more than limited ATP production by anaerobic glycolysis, is a key factor affecting recovery on subsequent reperfusion. In contrast to lactate efflux, purine and noradrenaline release are useful markers of ischemic and reperfusion damage. Kluwer Academic Publishers, 1996 ATP breakdown nationallicence catecholamine nationallicence glycogen nationallicence ischemia nationallicence Cargnoni Anna Fondazione Clinica del Lavoro, Centro di Fisiopatologia Cardiovascolare ‘Salvatore Maugeri,', Gussago, Brescia, Italy aut Ceconi Claudio Fondazione Clinica del Lavoro, Centro di Fisiopatologia Cardiovascolare ‘Salvatore Maugeri,', Gussago, Brescia, Italy aut Curello Salvatore Fondazione Clinica del Lavoro, Centro di Fisiopatologia Cardiovascolare ‘Salvatore Maugeri,', Gussago, Brescia, Italy aut Benigno Massimo Fondazione Clinica del Lavoro, Centro di Fisiopatologia Cardiovascolare ‘Salvatore Maugeri,', Gussago, Brescia, Italy aut de Jong Jan I Thoraxcentre, Erasmus University Rotterdam, The Netherlands aut Ferrari Roberto Cattedra di Cardiologia, Universita' degli Studi di Brescia, c/o Spedali Civili, P.le Spedali Civili, 1, 25123, Brescia, Italy aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 187-194 0300-8177 160-161:1<187 1996 160-161 11010 https://doi.org/10.1007/BF00240049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00240049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cargnoni Anna Fondazione Clinica del Lavoro, Centro di Fisiopatologia Cardiovascolare ‘Salvatore Maugeri,', Gussago, Brescia, Italy aut NATIONALLICENCE 700 1- Ceconi Claudio Fondazione Clinica del Lavoro, Centro di Fisiopatologia Cardiovascolare ‘Salvatore Maugeri,', Gussago, Brescia, Italy aut NATIONALLICENCE 700 1- Curello Salvatore Fondazione Clinica del Lavoro, Centro di Fisiopatologia Cardiovascolare ‘Salvatore Maugeri,', Gussago, Brescia, Italy aut NATIONALLICENCE 700 1- Benigno Massimo Fondazione Clinica del Lavoro, Centro di Fisiopatologia Cardiovascolare ‘Salvatore Maugeri,', Gussago, Brescia, Italy aut NATIONALLICENCE 700 1- de Jong Jan I Thoraxcentre, Erasmus University Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Ferrari Roberto Cattedra di Cardiologia, Universita' degli Studi di Brescia, c/o Spedali Civili, P.le Spedali Civili, 1, 25123, Brescia, Italy aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 187-194 0300-8177 160-161:1<187 1996 160-161 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer