caa a22 4500 477119670 CHVBK 20180405111632.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00240050 doi (NATIONALLICENCE)springer-10.1007/BF00240050 On the regulation of cellular energetics in health and disease [Elektronische Daten] [V. Saks, T. Tiivel, L. Kay, V Novel-Chaté, Z. Daneshrad, A. Rossi, E. Fontaine, C. Keriel, X. Leverve, R. Ventura-Clapier, K. Anflous, J. Samuel, L. Rappaport] Very recent experimental data, obtained by using the permeabilized cell technique or tissue homogenates for investigation of the mechanisms of regulation of respiration in the cells in vivo, are shortly summarized. In these studies, surprisingly high values of apparent Km for ADP, exceeding that for isolated mitochondria in vitro by more than order of magnitude, were recorded for heart, slow twitch skeletal muscle, hepatocytes, brain tissue homogenates but not for fast twitch skeletal muscle. Mitochondrial swelling in the hypo-osmotic medium resulted in the sharp decrease of the value of Km for ADP in correlation with the degree of rupture of mitochondrial outer membrane, as determined by the cytochrome c test. Very similar effect was observed when trypsin was used for treatment of skinned fibers, permeabilized cells or homogenates. It is concluded that, in many but not all types of cells, the permeability of the mitochondrial outer membrane for ADP is controlled by some cytoplasmic protein factor(s). Since colchicine and taxol were not found to change high values of the apparent Km for ADP, the participation of microtubular system seems to be excluded in this kind of control of respiration but studies of the roles of other cytoskeletal structures seem to be of high interest. In acute ischemia we observed rapid increase of the permeability of the mitochondrial outer membrane for ADP due to mitochondrial swelling and concomitant loss of creatine control of respiration as a result of dissociation of creatine kinase from the inner mitochondrial membrane. The extent of these damages was decreased by use of proper procedures of myocardial protection showing that outer mitochondrial membrane permeability and creatine control of respiration are valuable indices of myocardial preservation. In contrast to acute ischemia, chronic hypoxia seems to improve the cardiac cell energetics as seen from better postischemic recovery of phosphocreatine, and phosphocreatine overshoot after inotropic stimulation. In general, adaptational possibilities and pathophysiological changes in the mitochondrial outer membrane system point to the central role such a system may play in regulation of cellular energetics in vivo. KluwerAcademic Publishers, 1996 respiration nationallicence ADP diffusion nationallicence heart nationallicence skeletal muscle nationallicence liver nationallicence brain nationallicence in vivo regulation nationallicence Saks V. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut Tiivel T. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut Kay L. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut Novel-Chaté V. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut Daneshrad Z. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut Rossi A. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut Fontaine E. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut Keriel C. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut Leverve X. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut Ventura-Clapier R. Laboratoire de Cardiologie Cellulaire et Moleculaire INSERM Unité 446, Université Paris-Sud, Chatenay-Malabry aut Anflous K. Laboratoire de Cardiologie Cellulaire et Moleculaire INSERM Unité 446, Université Paris-Sud, Chatenay-Malabry aut Samuel J. Hôpital Lariboisiere, INSERM Unité 127, Paris, France aut Rappaport L. Hôpital Lariboisiere, INSERM Unité 127, Paris, France aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 195-208 0300-8177 160-161:1<195 1996 160-161 11010 https://doi.org/10.1007/BF00240050 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00240050 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saks V. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut NATIONALLICENCE 700 1- Tiivel T. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut NATIONALLICENCE 700 1- Kay L. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut NATIONALLICENCE 700 1- Novel-Chaté V. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut NATIONALLICENCE 700 1- Daneshrad Z. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut NATIONALLICENCE 700 1- Rossi A. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut NATIONALLICENCE 700 1- Fontaine E. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut NATIONALLICENCE 700 1- Keriel C. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut NATIONALLICENCE 700 1- Leverve X. Laboratories of Bioenergetics of Institute of Biological and Chemical Physics, Tallinn, Estonia aut NATIONALLICENCE 700 1- Ventura-Clapier R. Laboratoire de Cardiologie Cellulaire et Moleculaire INSERM Unité 446, Université Paris-Sud, Chatenay-Malabry aut NATIONALLICENCE 700 1- Anflous K. Laboratoire de Cardiologie Cellulaire et Moleculaire INSERM Unité 446, Université Paris-Sud, Chatenay-Malabry aut NATIONALLICENCE 700 1- Samuel J. Hôpital Lariboisiere, INSERM Unité 127, Paris, France aut NATIONALLICENCE 700 1- Rappaport L. Hôpital Lariboisiere, INSERM Unité 127, Paris, France aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 195-208 0300-8177 160-161:1<195 1996 160-161 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer