caa a22 4500 477119689 CHVBK 20180405111632.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00240057 doi (NATIONALLICENCE)springer-10.1007/BF00240057 Prevention by 7-oxo-prostacyclin of the calcium paradox in rat heart: Role of the sarcolemmal (Na,K)-ATPase [Elektronische Daten] [Attila Ziegelhöffer, Tatiana Ravingerová, Andrej Džurba, Narcisa Tribulová, Jan Slezák, Albert Breier, László Szekeres] It is demonstrated a fast and significant depression in the sarcolemmal (Na,K)-ATPase activity that occurs as early as 25 sec after the onset of Ca2+ depletion, and participates in the development of Ca2+-paradox in the rat heart. Pretreatment of the animals with 7-oxo-prostacyclin (PG12) 24-48 h prior to the experiment prevented fairly the Ca2+-depletion-induced depression in (Na,K)ATPase activity and the accompanying structural and functional damage to the heart and sarcolemma during Ca2+-depletion as well as the development of Ca2+-paradox during the subsequent Ca2+-repletion. Pretreatment with PGI, was chosen intentionally because previous experiments revealed, that in its late effect the drug is acting via stabilizing the membranes due induction of high activity of (Na,K)-ATPase that has increased affinity to ATP. From results obtained the following may be concluded: If during the phase of Ca2+-deprivation, the capability of heart sarcolemma to maintain sodium extrusion remains preserved, the expected aggravation of Ca2+-overload injury to Ca2+-paradox that would develop during Ca2+-repletion, may be definitely prevented. Sufficiently preserved (Na,K)-ATPase activity, hand in hand with stabilized sarcolemmal structure, may prevent an accumulation of sodium beneath the sarcolemma and consequently also an overexcessive entry of Ca2+ into the myocytes. Kluwer Academie Publishers, 1996 7-oxo-prostacyclin nationallicence calcium paradox nationallicence sarcolemmal (Na,K)-ATPase nationallicence subsarcolemmal sodium nationallicence subsarcolemmal Na/Ca ratio nationallicence calcium overload of the heart nationallicence Ziegelhöffer Attila Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, 842 33, Bratislava, USSR aut Ravingerová Tatiana Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, 842 33, Bratislava, USSR aut Džurba Andrej Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, 842 33, Bratislava, USSR aut Tribulová Narcisa Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, 842 33, Bratislava, USSR aut Slezák Jan Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, 842 33, Bratislava, USSR aut Breier Albert Institute of Molecular Physiology and Genetics, Bratislava, Slovak Republic aut Szekeres László Institute of Pharmacology, Albert Szent-Györgyi Medical University, Szeged, Hungary aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 257-263 0300-8177 160-161:1<257 1996 160-161 11010 https://doi.org/10.1007/BF00240057 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00240057 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ziegelhöffer Attila Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, 842 33, Bratislava, USSR aut NATIONALLICENCE 700 1- Ravingerová Tatiana Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, 842 33, Bratislava, USSR aut NATIONALLICENCE 700 1- Džurba Andrej Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, 842 33, Bratislava, USSR aut NATIONALLICENCE 700 1- Tribulová Narcisa Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, 842 33, Bratislava, USSR aut NATIONALLICENCE 700 1- Slezák Jan Institute for Heart Research, Slovak Academy of Sciences, Dúbravská cesta 9, 842 33, Bratislava, USSR aut NATIONALLICENCE 700 1- Breier Albert Institute of Molecular Physiology and Genetics, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Szekeres László Institute of Pharmacology, Albert Szent-Györgyi Medical University, Szeged, Hungary aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 160-161/1(1996-03-01), 257-263 0300-8177 160-161:1<257 1996 160-161 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer