caa a22 4500 477119751 CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00714333 doi (NATIONALLICENCE)springer-10.1007/BF00714333 Thermal response in murine L929 cells lacking αB-crystallin expression and αB-crystallin expressing L929 transfectants [Elektronische Daten] [Robert Blackburn, Sandra Galoforo, Christine Berns, Mark Ireland, Joong Cho, Peter Corry, Yong Lee] We investigated the role of αB-crystallin expression in the development of thermotolerance in murine L929 cells. An initial heat-shock of 10 min at 45°C induced thermotolerance in these cells to a heat challenge at 45°C administered 24 h later. The thermotolerance ratio at 10−1 isosurvival was 1.7. Expression of αB-crystallin gene was not detected during the 24 h incubation at 37°C following heat shock by either northern or western blots. In contrast, inducible HSP70 synthesis was observed during this time period. Thus, this cell line provided an unique system in which to examine the effects of transfected αB-crystallin on thermoresistance and thermotolerance. Cells stably transfected with αB-crystallin under the control of an inducible promoter did not show a significant increase in the ability to develop thermotolerance. However, a stably transfected L929 clone expressing high levels of constitutive αB-crystallin exhibited an approximately 50% increase in thermal resistance over parental and control cells. Though expression of αB-crystallin is not requisite for the development of thermotolerance in L929 cells, overexpression of transfected αB-crystallin can contribute to increased thermoresistance. Kluwer Academic Publishers, 1996 αB-Crystallin nationallicence transfection nationallicence thermotolerance nationallicence L929 cells nationallicence thermoresistance nationallicence Blackburn Robert Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut Galoforo Sandra aut Berns Christine Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut Ireland Mark Department ofAnatomy and Cell Biology, Wayne State University, 540 E. Canfield, 48201, Detroit, Michigan aut Cho Joong Lucky Biotech Corp., 5919 Hollis Street, 94608, Emeryville, California, USA aut Corry Peter Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut Lee Yong Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/1(1996-03-01), 51-60 0300-8177 155:1<51 1996 155 11010 https://doi.org/10.1007/BF00714333 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00714333 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Blackburn Robert Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut NATIONALLICENCE 700 1- Galoforo Sandra aut NATIONALLICENCE 700 1- Berns Christine Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut NATIONALLICENCE 700 1- Ireland Mark Department ofAnatomy and Cell Biology, Wayne State University, 540 E. Canfield, 48201, Detroit, Michigan aut NATIONALLICENCE 700 1- Cho Joong Lucky Biotech Corp., 5919 Hollis Street, 94608, Emeryville, California, USA aut NATIONALLICENCE 700 1- Corry Peter Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut NATIONALLICENCE 700 1- Lee Yong Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/1(1996-03-01), 51-60 0300-8177 155:1<51 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer