caa a22 4500 47711976X CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00714337 doi (NATIONALLICENCE)springer-10.1007/BF00714337 Expression of calcium-binding protein regucalcin mRNA in hepatoma cells [Elektronische Daten] [Reiko Makino, Masayoshi Yamaguchi] Whether the gene expression of hepatic Ca2+-binding protein regucalcin is altered in hepatomas was investigated. The change in regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin complementary DNA (0.9 kb). Rat hepatoma was induced by continuous feeding of basal diet containing 0.06% 3′-methyl-4-dimethylaminoazobenzene (3′-Me-DAB). After 35 weeks feeding, rats were sacrificed, and the non-tumorous and tumorous tissues of the livers were removed. In individual rats, the regucalcin mRNA levels in the tumorous tissues were generally decreased in comparison with that of the non-tumorous tissues of the chemical-fed rats, although the chemical administration might decrease the mRNA expression in normal rat liver, suggesting that the chemical administration causes a suppresive effect on the mRNA expression. When the genomic DNA extracted from the liver tumorous tissues was digested with restriction enzymes (EcoRI, BamHI and HindIII) and analyzed by Southern blotting, no rear-ranged band was found in the regucalcin gene from the hepatoma. Interestingly, in the transplantable Morris hepatoma cells, the regucalcin mRNA was markedly expressed, while the albumin mRNA was expressed only slightly. The present study demonstrates that regucalcin mRNA is clearly expressed in the transformed cells (Morris hepatoma cells). Kluwer Academic Publishers, 1996 regucalcin nationallicence calcium-binding protein nationallicence gene expression nationallicence rat hepatoma nationallicence Morris hepatoma cells nationallicence Makino Reiko Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut Yamaguchi Masayoshi Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/1(1996-03-01), 85-90 0300-8177 155:1<85 1996 155 11010 https://doi.org/10.1007/BF00714337 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00714337 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Makino Reiko Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut NATIONALLICENCE 700 1- Yamaguchi Masayoshi Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/1(1996-03-01), 85-90 0300-8177 155:1<85 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer