caa a22 4500 477119778 CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00714336 doi (NATIONALLICENCE)springer-10.1007/BF00714336 Preferential uptake of long chain polyunsaturated fatty acids by isolated human placental membranes [Elektronische Daten] [Fiona Campbell, Margaret Gordon, Asim Dutta-Roy] Fatty acid uptake by the placenta is thought to be a carrier-mediated process, however the mechanism by which long chain polyunsaturated fatty acids (LCPUFA) are preferentially accumulated from the maternal circulation to the fetal tissues is still unclear. To examine the role of the placenta in this process, binding of four different radiolabelled fatty acids ([14C]oleate, [14C]linoleate, [14C]a-linolenate and [14C]arachidonate) to human placental membranes was studied. Binding of fatty acid was found to be time- and temperature dependent. At equilibrium, the total binding of oleate was highest (5.1 ± 0.1 nmoles/mg protein) followed by linoleate (2.8 ± 0.31 nmoles/mg protein) and arachidonate (2.06 ± 0.4 nmoles/mg protein) and α-linolenate binding was lowest (0.5+0.1 nmoles/mg protein). However, oleate had the lowest specific binding (37% of the total binding) whereas arachidonate had the highest specific binding (∼ 86% of the total binding) followed by linoleate and a-linolenate (62%, and 69% of the total binding, respectively). Binding of each [14C] fatty acid was also assessed in the presence of 20-fold excess of other unlabelled ligands. Binding sites seem to have preference for the binding of [14C] fatty acids in the following order: arachidonic acid ⋙ linoleic acid ≫ a-linolenic acid ⋙≫ oleic acid, whereas BSP and a-tocopherol did not show any competition with any of the [14C] fatty acids. These data suggest that the fatty acid binding sites in placental membranes are specific for the fatty acids but that they have heterogeneous affinities.Trans fatty acids (elaidic and linoelaidic acids) also competed very strongly for the [14C] fatty acid binding. Polyclonal antiserum raised against placental FABPpm inhibited binding of these [14C]fatty acids but with variable degrees of inhibition; EFA/LCPUFA binding was much more than that of oleate. Our data suggest that EFA/LCPUFA bound to albumin are preferentially transported by human placental membranes and that the placental FABPpm may be involved in the sequestration of EFA/LCPUFA by the placenta. Kluwer Academic Publishers, 1996 human placenta nationallicence fatty acid uptake nationallicence essential fatty acids nationallicence plasma membrane fatty acid-binding protein (FABPpm nationallicence long chain polyunsaturated fatty acids nationallicence fetal development nationallicence Campbell Fiona Rowett Research Institute, AB2 9SB, Aberdeen, Scotland, UK aut Gordon Margaret Rowett Research Institute, AB2 9SB, Aberdeen, Scotland, UK aut Dutta-Roy Asim Rowett Research Institute, AB2 9SB, Aberdeen, Scotland, UK aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/1(1996-03-01), 77-83 0300-8177 155:1<77 1996 155 11010 https://doi.org/10.1007/BF00714336 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00714336 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Campbell Fiona Rowett Research Institute, AB2 9SB, Aberdeen, Scotland, UK aut NATIONALLICENCE 700 1- Gordon Margaret Rowett Research Institute, AB2 9SB, Aberdeen, Scotland, UK aut NATIONALLICENCE 700 1- Dutta-Roy Asim Rowett Research Institute, AB2 9SB, Aberdeen, Scotland, UK aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/1(1996-03-01), 77-83 0300-8177 155:1<77 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer