caa a22 4500 477119808 CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00714334 doi (NATIONALLICENCE)springer-10.1007/BF00714334 Effect of interleukin-1 receptor antagonist (IL-1RA) on histamine and serotonin release by rat basophilic leukemia cells (RBL-2H3) and peritoneal mast cells [Elektronische Daten] [Pio Conti, William Bouchet, Claudio Feliciani, Sandra Mammarella, Leena Kudchadker, Renato Barbacane, Marcella Reale, Iman Haggag, Gilles Bruneau, Jean Thibault, Theoharis Theoharides] Recently, it has been appreciated that cultured mast cells are significant sources of cytokines. However, the role of interkeukin-1 (IL-1) on mast cells and/or basophil degranulation is still unclear. In this report we provide evidence that rat basophilic leukemia cells (RBLC) cultured with a natural inhibitor of IL-1, interleukin-1 receptor antagonist (IL-1RA) (500 ng/ml) for 48 h, strongly inhibited the spontaneous release of serotonin (5HT) and histamine (from 22.50 to 43.49%), compared to untreated cells (control). When IL-1RA-treated and untreated RBLC were stimulated with a secretagogue (anti-IgE), no difference was found in the percent of 5HT and histamine release. Moreover, in another set of experiments using rat peritoneal mast cells (RPMC) treated and untreated with IL-1RA, we found that IL-1RA did not affect the release of 5HT or histamine, even when the secretagogue anti-IgE or compound 48/80 (C48/80) were used. The present studies describe an additional biological activity of IL-1RA, inhibiting histamine and 5HT release from RBLC cultures. Kluwer Academic Publishers, 1996 interleukin-1 receptor antagonist nationallicence histamine nationallicence serotonin nationallicence mast cells nationallicence rat basophilic leukemia cells nationallicence IL-1 : interleukin-1 nationallicence RA : receptor antagonist nationallicence 5HT : serotonin nationallicence RBLC : rat basophilic leukemia cells nationallicence RPMC : rat peritoneal mast cells nationallicence IgE : immunoglobulin E nationallicence FcΣ : immunoglobulin E receptor nationallicence CPM : counts per minute nationallicence BSA : bovine serum albumin nationallicence C48/80 : compound 48/80 nationallicence TNF : tumor necrosis factor nationallicence Conti Pio Immunology Division, Institute of Experimental Medicine, University of Chieti, Via dei Vestini, 66100, Chieti, Italy aut Bouchet William Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, 02111, Boston, Massachusetts, USA aut Feliciani Claudio Dermatology Division, University of Chieti, Via dei Vestini, 66100, Chieti, Italy aut Mammarella Sandra Dermatology Division, University of Chieti, Via dei Vestini, 66100, Chieti, Italy aut Kudchadker Leena Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, 02111, Boston, Massachusetts, USA aut Barbacane Renato Immunology Division, Institute of Experimental Medicine, University of Chieti, Via dei Vestini, 66100, Chieti, Italy aut Reale Marcella Immunology Division, Institute of Experimental Medicine, University of Chieti, Via dei Vestini, 66100, Chieti, Italy aut Haggag Iman Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, 02111, Boston, Massachusetts, USA aut Bruneau Gilles Laboratoire de Biochimie Cellulaire, College de France, Paris, France aut Thibault Jean Laboratoire de Biochimie Cellulaire, College de France, Paris, France aut Theoharides Theoharis Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, 02111, Boston, Massachusetts, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/1(1996-03-01), 61-68 0300-8177 155:1<61 1996 155 11010 https://doi.org/10.1007/BF00714334 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00714334 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Conti Pio Immunology Division, Institute of Experimental Medicine, University of Chieti, Via dei Vestini, 66100, Chieti, Italy aut NATIONALLICENCE 700 1- Bouchet William Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, 02111, Boston, Massachusetts, USA aut NATIONALLICENCE 700 1- Feliciani Claudio Dermatology Division, University of Chieti, Via dei Vestini, 66100, Chieti, Italy aut NATIONALLICENCE 700 1- Mammarella Sandra Dermatology Division, University of Chieti, Via dei Vestini, 66100, Chieti, Italy aut NATIONALLICENCE 700 1- Kudchadker Leena Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, 02111, Boston, Massachusetts, USA aut NATIONALLICENCE 700 1- Barbacane Renato Immunology Division, Institute of Experimental Medicine, University of Chieti, Via dei Vestini, 66100, Chieti, Italy aut NATIONALLICENCE 700 1- Reale Marcella Immunology Division, Institute of Experimental Medicine, University of Chieti, Via dei Vestini, 66100, Chieti, Italy aut NATIONALLICENCE 700 1- Haggag Iman Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, 02111, Boston, Massachusetts, USA aut NATIONALLICENCE 700 1- Bruneau Gilles Laboratoire de Biochimie Cellulaire, College de France, Paris, France aut NATIONALLICENCE 700 1- Thibault Jean Laboratoire de Biochimie Cellulaire, College de France, Paris, France aut NATIONALLICENCE 700 1- Theoharides Theoharis Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, 02111, Boston, Massachusetts, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/1(1996-03-01), 61-68 0300-8177 155:1<61 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer