caa a22 4500 477119832 CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00714328 doi (NATIONALLICENCE)springer-10.1007/BF00714328 Matrix metalloproteinase activity expression in infarcted, noninfarcted and dilated cardiomyopathic human hearts [Elektronische Daten] [Suresh Tyagi, Scott Campbell, Hanumanth Reddy, Eko Tjahja, Donald Welker] In the normal myocardium matrix metalloproteinases (MMP) are present in the latent form. To examine whether MMP are activated following infarction or idiopathic dilated cardiomyopathy (DCM), we extracted and measured MMP activity in tissue derived from 7 explanted, failing human hearts due to either previous myocardial infarction (MI) or DCM. MMP activity in infarcted left ventricle (LV), noninfarcted IV and right ventricle (RV) from MI patients, as well as tissue from either ventricle of DCM patients, were compared to the activity of donor heart tissue. SDS-PAGE and dye-binding assays were used to determine total protein concentration, while collagenase activity was measured by SDS-PAGE type substrate gels embedded with type I gelatin (zymography). Accuracy of the zymographic technique was shown for tissue samples as small as 0.05 mg and was comparable to results obtained by a spectrophotometric method.. After normalization for total protein concentration, we found 3 ± 1 % collagenase activity in normal atrial tissue which could be activated to 80-90% by trypsin or plasmin, indicating that collagenase is normally inactive or in a latent form in human heart. In endo- and epimyocardium of infarcted LV on the other hand, collagenase activity was 85-95% and 10-20%, respectively, while 5-10% and 3-5%, respectively, in noninfarcted LV In DCM, collagenolytic activity in the endo and epimyocardium was 75 ± 5 and 35 ± 5% in the LV and 35 ± 7 and 20 ± 5% in the RV, respectively. Thus, in dilated failing human hearts secondary to previous MI or DCM, MMP activity is increased. This is particularly the case within the endomyocardium of the infarcted and noninfarcted portions of either ventricle with MI and in both ventricles in DCM. This suggests that an activation of collagenase throughout the myocardium may contribute to its remodeling that includes ventricular dilatation and wall thinning. KluwerAcademic Publishers, 1996 collagenase nationallicence infarction nationallicence dilatation nationallicence human heart nationallicence matrix metalloproteinase nationallicence cardiomyopathy nationallicence myocardium nationallicence Tyagi Suresh Departments of Internal Medicine and of Biochemistry and Dalton Cardiovascular Research Center, University of Missouri, Health Sciences Center, 65212, Columbia, MO, USA aut Campbell Scott Departments of Internal Medicine and of Biochemistry and Dalton Cardiovascular Research Center, University of Missouri, Health Sciences Center, 65212, Columbia, MO, USA aut Reddy Hanumanth Departments of Internal Medicine and of Biochemistry and Dalton Cardiovascular Research Center, University of Missouri, Health Sciences Center, 65212, Columbia, MO, USA aut Tjahja Eko Departments of Internal Medicine and of Biochemistry and Dalton Cardiovascular Research Center, University of Missouri, Health Sciences Center, 65212, Columbia, MO, USA aut Welker Donald Departments of Internal Medicine and of Biochemistry and Dalton Cardiovascular Research Center, University of Missouri, Health Sciences Center, 65212, Columbia, MO, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/1(1996-03-01), 13-21 0300-8177 155:1<13 1996 155 11010 https://doi.org/10.1007/BF00714328 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00714328 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tyagi Suresh Departments of Internal Medicine and of Biochemistry and Dalton Cardiovascular Research Center, University of Missouri, Health Sciences Center, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Campbell Scott Departments of Internal Medicine and of Biochemistry and Dalton Cardiovascular Research Center, University of Missouri, Health Sciences Center, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Reddy Hanumanth Departments of Internal Medicine and of Biochemistry and Dalton Cardiovascular Research Center, University of Missouri, Health Sciences Center, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Tjahja Eko Departments of Internal Medicine and of Biochemistry and Dalton Cardiovascular Research Center, University of Missouri, Health Sciences Center, 65212, Columbia, MO, USA aut NATIONALLICENCE 700 1- Welker Donald Departments of Internal Medicine and of Biochemistry and Dalton Cardiovascular Research Center, University of Missouri, Health Sciences Center, 65212, Columbia, MO, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/1(1996-03-01), 13-21 0300-8177 155:1<13 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer