caa a22 4500 477119875 CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00229311 doi (NATIONALLICENCE)springer-10.1007/BF00229311 Superinduction of mouse epidermal ornithine decarboxylase activity by repeated 12-0-tetradecanoylphorbol-13-acetate treatments [Elektronische Daten] [A.K. Verma, K.M. Hsiao, H. Ahrens, M. Suganuma, H. Fujiki, S. Matsufuji, H. Hayashi] A correlation of the levels of epidermal protein kinase C (PKC) isozymes, steady state levels of ornithine decarboxylase (ODC) mRNA, and ODC antizyme with the induction of ornithine decarboxylase (ODC) activity by a second repeat 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment to mouse skin was determined. A single application of TPA to female CD-1 mouse skin leads to a dramatic induction of ODC activity (≈3 nmol CO2/60 min/mg protein) which peaks at about 5 h after treatment. However, a superinduction of ODC activity (≈13 CO2/60 min/mg protein) is observed upon the second TPA application at 48 or 72 h after the first TPA treatment. Prior application of a tumor initiating dose of 7,12-dimethylbenz[a]anthracine to mouse skin did not influence the degree of induction of ODC by a repeat TPA treatment. Western Blot analyses using antibodies specific to PKC α, β, γ, ɛ and s indicate detectable levels of PKC α, β, δ and ε in mouse epidermal extracts. A time course of the effects of a single topical application of 20 nmol of TPA to the mouse skin indicate that none of PKC isozymes (α, β, γ, δ and ɛ) were completely downregulated at times (72 h) when ODC was overinduced by TPA. TPA-induced steady state levels of ODC mRNA did not correlate with the degree of superinduction of ODC activity by TPA. The second TPA treatment, 72 h after the first TPA treatment, which leads to superinduction of ODC activity did not decrease the levels of the ODC-antizyme. The results indicate that superinduction of mouse epidermal ODC activity is regulated in part post-transcriptionally and may not be the result of either a loss of PKC isoform(s) or a decrease in the levels of ODC antizyme. Kluwer Academic Publishers, 1996 ODC nationallicence PKC nationallicence tumor promotion nationallicence TPA : 12-0-tetradecanoylphorbol-l3-acetate nationallicence PKC : protein kinase C nationallicence ODC : ornithine decarboxylase nationallicence IPTG : Isopropyl β-D-thiogalactopyranoside nationallicence Verma A.K. Department of Human Oncology, Medical School, University of Wisconsin Comprehensive Cancer Center, USA aut Hsiao K.M. Department of Human Oncology, Medical School, University of Wisconsin Comprehensive Cancer Center, USA aut Ahrens H. Department of Human Oncology, Medical School, University of Wisconsin Comprehensive Cancer Center, USA aut Suganuma M. National Cancer Research Institute, Jikei University School of Medicine, Tokyo, Japan aut Fujiki H. National Cancer Research Institute, Jikei University School of Medicine, Tokyo, Japan aut Matsufuji S. Jikei University School of Medicine, Tokyo, Japan aut Hayashi H. Jikei University School of Medicine, Tokyo, Japan aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 139-151 0300-8177 155:2<139 1996 155 11010 https://doi.org/10.1007/BF00229311 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229311 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Verma A.K. Department of Human Oncology, Medical School, University of Wisconsin Comprehensive Cancer Center, USA aut NATIONALLICENCE 700 1- Hsiao K.M. Department of Human Oncology, Medical School, University of Wisconsin Comprehensive Cancer Center, USA aut NATIONALLICENCE 700 1- Ahrens H. Department of Human Oncology, Medical School, University of Wisconsin Comprehensive Cancer Center, USA aut NATIONALLICENCE 700 1- Suganuma M. National Cancer Research Institute, Jikei University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Fujiki H. National Cancer Research Institute, Jikei University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsufuji S. Jikei University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Hayashi H. Jikei University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 139-151 0300-8177 155:2<139 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer