caa a22 4500 477119891 CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00229313 doi (NATIONALLICENCE)springer-10.1007/BF00229313 Hypoglycemia-induced AP-1 transcription factor and basic fibroblast growth factor gene expression in multidrug resistant human breast carcinoma MCF-7/ADR cells [Elektronische Daten] [Sandra Galoforo, Christine Berns, Geza Erdos, Peter Corry, Yong Lee] We investigated the effect of hypoglycemic treatment on the activation of the AP-1 transcription factors and the regulation of basic fibroblast growth factor (bFGF) gene expression in multidrug resistant human breast carcinoma MCF-7/ADR cells. Northern blot and gel mobility shift assays showed that hypoglycemic treatment induced c-jun and c-fos gene expression, AP-1 binding activity, as well as bFGF gene expression. Moreover, transfected cells expressing high levels of abnormal c-Jun protein exhibited a reduction in the bFGF protein levels compared to parental cells. A potent protein kinase C (PKC) inhibitor, H-7 (60 μg/ml) suppressed the stress-induced bFGF gene expression. Our study also demonstrated that H-7 did not facilitate the decay of bFGF mRNA. Thus, the suppression of bFGF gene expression by treatment with H-7 was due to the effect of the drug on the synthesis of bFGF mRNA rather than the stability of bFGF mRNA. Our data suggest that hypoglycemia-induced bFGF gene expression is mediated through the activation of PKC and the AP-1 transcription factors. (Mol Cell Biochem 155: 163-171, 1996) Kluwer Academic Publishers, 1996 AP-1 binding activity nationallicence basic fibroblast growth factor nationallicence c-Jun mutant protein nationallicence H-7 nationallicence Galoforo Sandra Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut Berns Christine Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut Erdos Geza Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut Corry Peter Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut Lee Yong Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 163-171 0300-8177 155:2<163 1996 155 11010 https://doi.org/10.1007/BF00229313 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229313 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Galoforo Sandra Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut NATIONALLICENCE 700 1- Berns Christine Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut NATIONALLICENCE 700 1- Erdos Geza Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut NATIONALLICENCE 700 1- Corry Peter Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut NATIONALLICENCE 700 1- Lee Yong Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 163-171 0300-8177 155:2<163 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer