caa a22 4500 477119905 CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00229310 doi (NATIONALLICENCE)springer-10.1007/BF00229310 Characterization of the HeLa Cell 35 kDa Alu-element binding protein [Elektronische Daten] [Yungping Chiang, Jamboor Vishwanatha] HumanAlu-elements are short interspersed DNA sequences that comprise approximately 5% of the human genome. The physiological role of Alu-elements are unknown, although they are proposed to be involved in DNA replication, transcriptional regulation and nuclear transport of signal recognition particle RNA. Proteins that bind to Alu-element and Alu RNA have been identified in human cells. In HeLa cells, two proteins of 120 kDa and 35 kDa specifically bind to Alu-elements. We find that the 35 kDa protein is localized exclusively to the nucleus, while the 120 kDa protein is distributed between nucleus and cytoplasm. The 35 kDa protein is regulated by phosphorylation. Upon dephosphorylation, its DNA-binding activity is significantly enhanced. Contrary to the recent identification of the smaller Alu-element binding protein as annexin 11, we find that annexin II is not an Alu-element binding protein. Using a variety of techniques, we demonstrate that the 35 kDa Alu-element binding protein is distinct from annexin II. Kluwer Academic Publishers, 1996 Alu element nationallicence Alu-element binding protein nationallicence DNA-binding protein nationallicence Annexin II, phosphorylation nationallicence Chiang Yungping The Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 600, S. 42nd Street, 68198-4525, Omaha, Nebraska, USA aut Vishwanatha Jamboor The Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 600, S. 42nd Street, 68198-4525, Omaha, Nebraska, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 131-138 0300-8177 155:2<131 1996 155 11010 https://doi.org/10.1007/BF00229310 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229310 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chiang Yungping The Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 600, S. 42nd Street, 68198-4525, Omaha, Nebraska, USA aut NATIONALLICENCE 700 1- Vishwanatha Jamboor The Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 600, S. 42nd Street, 68198-4525, Omaha, Nebraska, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 131-138 0300-8177 155:2<131 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer