caa a22 4500 477119913 CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00229307 doi (NATIONALLICENCE)springer-10.1007/BF00229307 Steroid hormonal regulation of calcium-binding protein regucalcin mRNA expression in the kidney cortex of rats [Elektronische Daten] [Hideyuki Kurota, Masayoshi Yamaguchi] The effect of various steroid hormones on the expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using rat liver regucalcin complementary DNA (0.9 kb of open-reading frame). Regucalcin mRNA was expressed in the kidney cortex but not the medulla. Rats received a single subcutaneous administration of steroid; the animals were sacrificed 60 min after the treatment of aldosterone (2.5, 5.0 and 10 μg/100 g body weight) or 6 h after the treatment of estrogen (17β-estradiol; 0.05, 0.1 and 0.2 mg/100 g), hydrocortisone (0.5, 1.0 and 3.0 mg/100 g) and dexamethasone (50, 100 and 150 μg/100 g). Regucalcin mRNA levels in the kidney cortex were clearly diminished by the administration of aldosterone or estrogen, while hydrocortisone administration had no effect. The administration of dexamethasone (100 μg/100 g) caused a remarkable increase of regucalcin mRNA levels in the kidney cortex. The dexamethasone-induced increase in regucalcin mRNA levels was completely blocked by the simultaneous administration of cycloheximide (150 μg/100 g), although the drug administration had no effect on the mRNA levels in control rats. Meanwhile, the dexamethasone administration did not cause an appreciable alteration of calcium content in the kidney cortex. The present study demonstrates that, of the various steroid hormones used, dexamethasone uniquely has a stimulatory effect on regucalcin mRNA expression in the kidney cortex of rats. The steroid effect may be mediated through a newly synthesized protein. Kluwer Academic Publishers, 1996 regucalcin nationallicence calcium-binding protein nationallicence aldosterone nationallicence estrogen nationallicence dexamethasone nationallicence gene expression nationallicence rat kidney cortex nationallicence Kurota Hideyuki Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City 422, Japan aut Yamaguchi Masayoshi Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City 422, Japan aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 105-111 0300-8177 155:2<105 1996 155 11010 https://doi.org/10.1007/BF00229307 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229307 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kurota Hideyuki Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City 422, Japan aut NATIONALLICENCE 700 1- Yamaguchi Masayoshi Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City 422, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 105-111 0300-8177 155:2<105 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer