caa a22 4500 477119921 CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00229314 doi (NATIONALLICENCE)springer-10.1007/BF00229314 Analysis of mRNA expression and cloning of a novel plasma membrane Ca2+-ATPase splice variant in human heart [Elektronische Daten] [Juan Santiago-García, Jaime Mas-Oliva, Dolores Saavedra, Angel Zarain-Herzberg] Four different plasma membrane CA2+-ATPase (PMCA) genes and three sarco(endo)plasmic reticulum Ca 2+-ATPase (SERCA) genes have been previously cloned and characterized. In this study we have investigated the expression of the mRNA encoding the various PMCA and SERCA proteins in fetal and adult human heart and placenta by the reverse-transcriptase-polymerase chain-reaction (RT PCR) and cDNA cloning. We have found that PMCA1 and PMCA4 genes were expressed in 8-, 12- and 20-week fetal heart and in adult heart. PMCA2 gene was expressed at low levels in adult heart but was not detected in fetal heart. PMCA3 mRNA was not detected in the heart nor placenta. In contrast, the mRNA encoding SERCA2a, SERCA2b and SERCA3 were expressed in all cardiac developmental stages. Multiple alternatively spliced mRNA transcripts which differ at splice site A and B/C of the PMCA 1, PMCA2 and PMCA4 genes were detected in the human heart. Interestingly, a novel tissue specific variant of the PMCA4 gene was detected in both fetal and adult human heart but not in placenta that accounts for about 30% of the total PMCA4 mRNA variant expression. DNA sequence analysis of this novel variant revealed that it corresponds to the equivalent of the PMCA 1 d variant and accordingly we have named it PMCA4d. We cloned and sequenced eight cDNA inserts encoding for the PMCA 1 and PMCA4 variants from a fetal human heart cDNA library confirming that these are the two main PMCA genes expressed in cardiac muscle. Kluwer Academic Publishers, 1996 development nationallicence cardiac muscle nationallicence placenta nationallicence sarcoplasmic reticulum nationallicence sarcolemma nationallicence plasma membrane nationallicence PMCA nationallicence SERCA2 nationallicence SERCA3 nationallicence cDNA cloning nationallicence PCR nationallicence Santiago-García Juan Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, and Department of Physiology, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut Mas-Oliva Jaime Departmento de Bioenergética, Instituto de Fisiologia Celular, Universidad Nacional Autonóma de México, Apartado Postal 70-243, 04510, D.F. aut Saavedra Dolores Hospital General Dr. Manuel Gea Gonzalez, Calzada de Tlalpan 4800, D.F, 14000, México aut Zarain-Herzberg Angel Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, and Department of Physiology, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 173-182 0300-8177 155:2<173 1996 155 11010 https://doi.org/10.1007/BF00229314 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229314 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santiago-García Juan Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, and Department of Physiology, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 700 1- Mas-Oliva Jaime Departmento de Bioenergética, Instituto de Fisiologia Celular, Universidad Nacional Autonóma de México, Apartado Postal 70-243, 04510, D.F aut NATIONALLICENCE 700 1- Saavedra Dolores Hospital General Dr. Manuel Gea Gonzalez, Calzada de Tlalpan 4800, D.F, 14000, México aut NATIONALLICENCE 700 1- Zarain-Herzberg Angel Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, and Department of Physiology, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 173-182 0300-8177 155:2<173 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer