caa a22 4500 47711993X CHVBK 20180405111633.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00229308 doi (NATIONALLICENCE)springer-10.1007/BF00229308 Fatty acid double bond orientation alters interaction with L-cell fibroblasts [Elektronische Daten] [Clayton Heyliger, Talat Kheshgi, Eric Murphy, Sean Myers-Payne, Friedhelm Schroeder] Relatively little is known of fatty acid specificity in cellular fatty acid uptake. In this study L-cells, a fibroblastic cell line with very low levels of endogenous cytosolic fatty acid binding protein, were used to examine the role of cis and trans unsaturation on fatty acid uptake. The fluorescent fatty acids, trans-parinaric acid and cis-parinaric acid, were used as analogs of straight-chain saturated, and kinked-chain unsaturated fatty acids, respectively, in order to evaluate the fatty acid specificity of the uptake system. Parinaric acid is poorly metabolizable; greater than 97% was unesterified while 3H-oleic acid was almost totally metabolized after 30 min uptake. Cis- and trans-parinaric acid uptake was saturable and dependent on the concentration of fatty acid. However, the initial rate and maximal amount of trans-parinaric acid taken up by the L-cells was greater than for cis-parinaric acid under the same conditions. The affinity of L-cell uptake for trans-parinaric acid (Km = 0.12 uM) was 35-fold higher than that for cis-parinaric acid (Km = 4.17 uM) . Based on competition studies with oleic and stearic acids, it was concluded that the cis- and trans-parinaric acid were taken up by the same L-cell fatty acid uptake system. The results suggest that the L-cell fatty acid uptake system has selectivity for straight chain rather than kinked chain unsaturated fatty acids. Kluwer Academic Publishers, 1996 L-cells nationallicence oleic acid nationallicence cis-parinaric acid nationallicence trans-parinaric acid nationallicence fatty acid nationallicence transport nationallicence fluorescence nationallicence Cis-parinaric acid : 9Z, 11E, 13E, 15Z-octatetraenoic acid nationallicence trans-parinaric acid : 9E, I IE, 13E, 15E-octatetraenoic acid nationallicence EGTA : ethylene glycol-bis(beta-amlno-ethyl ether) N,N,N′,N′-tetratacetic acid nationallicence BSA : bovine serum albumin nationallicence PBS : phosphate buffered saline nationallicence Heyliger Clayton Division of Pharmacology and Medical Chemistry, Department of Pharmacology & Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, OH aut Kheshgi Talat Division of Pharmacology and Medical Chemistry, Department of Pharmacology & Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, OH aut Murphy Eric Department of Physiology and Pharmacology, Texas A&M University, TVMC, 77843-4466, College Station, TX, USA aut Myers-Payne Sean Department of Physiology and Pharmacology, Texas A&M University, TVMC, 77843-4466, College Station, TX, USA aut Schroeder Friedhelm Department of Physiology and Pharmacology, Texas A&M University, TVMC, 77843-4466, College Station, TX, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 113-119 0300-8177 155:2<113 1996 155 11010 https://doi.org/10.1007/BF00229308 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229308 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heyliger Clayton Division of Pharmacology and Medical Chemistry, Department of Pharmacology & Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, OH aut NATIONALLICENCE 700 1- Kheshgi Talat Division of Pharmacology and Medical Chemistry, Department of Pharmacology & Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, OH aut NATIONALLICENCE 700 1- Murphy Eric Department of Physiology and Pharmacology, Texas A&M University, TVMC, 77843-4466, College Station, TX, USA aut NATIONALLICENCE 700 1- Myers-Payne Sean Department of Physiology and Pharmacology, Texas A&M University, TVMC, 77843-4466, College Station, TX, USA aut NATIONALLICENCE 700 1- Schroeder Friedhelm Department of Physiology and Pharmacology, Texas A&M University, TVMC, 77843-4466, College Station, TX, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 155/2(1996-02-01), 113-119 0300-8177 155:2<113 1996 155 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer