caa a22 4500 477120008 CHVBK 20180405111634.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00227891 doi (NATIONALLICENCE)springer-10.1007/BF00227891 Expression and regulation of chloride channels in neonatal rat cardiomyocytes [Elektronische Daten] [Ben C. Tilly, Karel Bezstarosti, Wendy E.M. Boomaars, Christopher R. Marino, Jos M.J. Lamers, Hugo de R.] Using an 125I− efflux assay, we have studied the expression of various types of chloride channels in isolated neonatal rat cardiomyocytes. Three different classes of anion conductances were distinguished: (1) a Cal2+-sensitive Cl− conductance, triggered upon stimulation of the cells with endothelin-1 or Ca2+-ionophore; (2) a CAMP/protein kinase A-operated Cl− conductance, activated by addition of forskolin. This anion channel could be identified as the Cystic Fibrosis Transmembrane conductance Regulator (CFTR-CI− channel) by Western blotting as well as by its enhanced activity in cultures pretreated with the tyrosine kinase inhibitor genistein; (3) a distinct class of cell volume-regulated Cl− channels, potentiated in the presence of endothelin-1 or the phosphotyrosine phosphatase inhibitor pervanadate. The potential role of each class of Cl− channels in the generation and/or modulation of action potentials as well as in maintaining cell volume is discussed. Kluwer Academic Publishers, 1996 cardiomyocytes nationallicence CFTR nationallicence chloride channels nationallicence cystic fibrosis nationallicence endothelin-1 nationallicence regulatory volume decrease nationallicence C. Tilly Ben Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands aut Bezstarosti Karel Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands aut E.M. Boomaars Wendy Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands aut R. Marino Christopher Department of Veterans Affairs Medical Center, Memphis, Tennessee, USA aut M.J. Lamers Jos Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands aut de R. Hugo Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 157/1-2(1996-04-01), 129-135 0300-8177 157:1-2<129 1996 157 11010 https://doi.org/10.1007/BF00227891 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227891 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- C. Tilly Ben Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands aut NATIONALLICENCE 700 1- Bezstarosti Karel Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands aut NATIONALLICENCE 700 1- E.M. Boomaars Wendy Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands aut NATIONALLICENCE 700 1- R. Marino Christopher Department of Veterans Affairs Medical Center, Memphis, Tennessee, USA aut NATIONALLICENCE 700 1- M.J. Lamers Jos Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands aut NATIONALLICENCE 700 1- de R Hugo Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, the Netherlands aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 157/1-2(1996-04-01), 129-135 0300-8177 157:1-2<129 1996 157 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer