caa a22 4500 477120032 CHVBK 20180405111634.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00227901 doi (NATIONALLICENCE)springer-10.1007/BF00227901 Metabolism of linoleic and α-linolenic acids in cultured cardiomyocytes: Effect of different N-6 and N-3 fatty acid supplementation [Elektronische Daten] [Alessandra Bordoni, Josè Lopez-Jimenez, Christian Spanò, PierLuigi Biagi, David Horrobin, Silvana Hrelia] The metabolites of linoleic (LA) and α-linolenic (ALA) acids are involved in coronary heart disease. Both n-6 and n-3 essential fatty acids (EFAs) are likely to be important in prevention of atherosclerosis since the common risk factors are associated with their reduced 6-desaturation. We previously demonstrated the ability of heart tissue to desaturate LA. In this study we examined the ability of cultured cardiomyocytes to metabolize both LA and ALA in vivo, in the absence and in the presence of gamma linolenic acid (GLA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) alone or combined together. In control conditions, about 25% of LA and about 90% of ALA were converted in PUFAs. GLA supplementation had no influence on LA conversion to more unsaturated fatty acids, while the addition of n-3 fatty acids, alone or combined together, significantly decreased the formation of interconversion products from LA. Using the combination of n-6 and n-3 PUFAs, GLA seemed to counterbalance partially the inhibitory effect of EPA and DHA on LA desaturation/elongation. The conversion of ALA to more unsaturated metabolites was greatly affected by GLA supplementation. Each supplemented fatty acid was incorporated to a significant extent into cardiomyocyte lipids, as revealed by gas chromatographic analysis. The n-6/n-3 fatty acid ratio was greatly influenced by the different supplementations; the ratio in GLA+EPA+DHA supplemented cardiomyocytes was the most similar to that recorded in control cardiomyocytes. Since important risk factors for coronary disease may be associated with reduced 6-desaturation of the parent EFAs, administration of n-6 or n-3 EFA metabolites alone could cause undesirable effects. Since they appear to have different and synergistic roles, only combined treatment with both n-6 and n-3 metabolites is likely to achieve optimum results. Kluwer Academic Publishers, 1996 essential fatty acids nationallicence polyunsaturated fatty acids nationallicence desaturation nationallicence cultured cardiomyocytes nationallicence heart disease nationallicence Bordoni Alessandra Department of Biochemistry ‘G. Moruzzi', University of Bologna, Bologna, Italy aut Lopez-Jimenez Josè Department of Biochemistry ‘G. Moruzzi', University of Bologna, Bologna, Italy aut Spanò Christian Department of Biochemistry ‘G. Moruzzi', University of Bologna, Bologna, Italy aut Biagi PierLuigi Department of Biochemistry ‘G. Moruzzi', University of Bologna, Bologna, Italy aut Horrobin David Efamol Research Institute, Kentville, Nova Scotia, Canada aut Hrelia Silvana Department of Biochemistry ‘G. Moruzzi', University of Bologna, Bologna, Italy aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 157/1-2(1996-04-01), 217-222 0300-8177 157:1-2<217 1996 157 11010 https://doi.org/10.1007/BF00227901 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227901 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bordoni Alessandra Department of Biochemistry ‘G. Moruzzi', University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Lopez-Jimenez Josè Department of Biochemistry ‘G. Moruzzi', University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Spanò Christian Department of Biochemistry ‘G. Moruzzi', University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Biagi PierLuigi Department of Biochemistry ‘G. Moruzzi', University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Horrobin David Efamol Research Institute, Kentville, Nova Scotia, Canada aut NATIONALLICENCE 700 1- Hrelia Silvana Department of Biochemistry ‘G. Moruzzi', University of Bologna, Bologna, Italy aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 157/1-2(1996-04-01), 217-222 0300-8177 157:1-2<217 1996 157 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer