caa a22 4500 477120229 CHVBK 20180405111634.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00227897 doi (NATIONALLICENCE)springer-10.1007/BF00227897 Regulation of expression of contractile proteins with cardiac hypertrophy and failure [Elektronische Daten] [Xavier Martin, Dylan Wynne, Peter Glennon, Anton Moorman, Kenneth Boheler] Transitions in sarcomeric α-actin and cardiac myosin heavy chain (MHC) gene expression have been useful as molecular markers for the development of cardiac hypertrophy and failure. In simpler model systems, α-actin expression has been useful in delineating some of the molecular pathways responsible for its induction following growth stimulation in vitro. In this study, we report that the effects of adrenergic agonists on α-actin expression in neonatal cardiocytes is dependent upon the culture conditions. In cardiocytes plated at 5 x 104 cells/cm2, skeletal α-actin mRNA levels represent 47%, 37% or 42% of total sarcomeric α-actin accumulations following administrations of 4 μM norepinephrine (NE), isoproterenol (Iso), or phenylephrine (PE), respectively. Cultured cardiocytes treated with vehicle (ascorbate) only accumulated 19% skeletal α-actin. Under these tissue culture conditions, in contrast to data reported previously, skeletal α-actin expression is regulated by both α- and β-adrenergic agonist stimulation. Furthermore, we present data showing that an endogenous anti-β-MHC transcript is regulated by both pressure-overload- or thyroxine-induced cardiac hypertrophy. Although anti-β-MHC transcripts do not play a major role in regulating β-MHC gene expression, the presence of this antisense transcript is associated with a novel set of β-MHC degradation products. In vitro studies, where oligonucleotides complementary to β-MHC have been introduced into cardiomyoctyes, show that the mRNA levels of β-MHC are decreased by 14-21 % within 72 h after addition of the oligonucleotides. This result together with the presence of β-MHC degradation products suggest that endogenous anti-β-MHC transcripts may be involved in a post-transcriptional regulatory mechanism affecting the steady-state levels of β-MHC expression. Kluwer Academic Publishers, 1996 sarcomeric α-actins nationallicence myosin heavy chains nationallicence neonatal rat cardiocytes nationallicence adrenergic agonists nationallicence antisense RNA nationallicence oligonucleotides nationallicence Martin Xavier Pavillon Rambuteau, Groupe Hospitalier Pitié-Salpetrière, INSERM U153, 47-83 Bd de l'Hopital, 75630, Paris Cedex 13, France aut Wynne Dylan National Heart and Lung Institute, the Departments of Cardiac Medicine Surgery, Imperial College, Dovehouse Street, SW3 6LY, London, UK aut Glennon Peter National Heart and Lung Institute, the Departments of Cardiothoracic Surgery, Imperial College, Dovehouse Street, SW3 6LY, London, UK aut Moorman Anton Department of Anatomy and Embryology, Academic Medical Centre, Meibergdreef 15, 1105, AZ Amsterdam, The Netherlands aut Boheler Kenneth National Heart and Lung Institute, the Departments of Cardiac Medicine Surgery, Imperial College, Dovehouse Street, SW3 6LY, London, UK aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 157/1-2(1996-04-01), 181-189 0300-8177 157:1-2<181 1996 157 11010 https://doi.org/10.1007/BF00227897 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227897 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martin Xavier Pavillon Rambuteau, Groupe Hospitalier Pitié-Salpetrière, INSERM U153, 47-83 Bd de l'Hopital, 75630, Paris Cedex 13, France aut NATIONALLICENCE 700 1- Wynne Dylan National Heart and Lung Institute, the Departments of Cardiac Medicine Surgery, Imperial College, Dovehouse Street, SW3 6LY, London, UK aut NATIONALLICENCE 700 1- Glennon Peter National Heart and Lung Institute, the Departments of Cardiothoracic Surgery, Imperial College, Dovehouse Street, SW3 6LY, London, UK aut NATIONALLICENCE 700 1- Moorman Anton Department of Anatomy and Embryology, Academic Medical Centre, Meibergdreef 15, 1105, AZ Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Boheler Kenneth National Heart and Lung Institute, the Departments of Cardiac Medicine Surgery, Imperial College, Dovehouse Street, SW3 6LY, London, UK aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 157/1-2(1996-04-01), 181-189 0300-8177 157:1-2<181 1996 157 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer