caa a22 4500 477120261 CHVBK 20180405111634.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00227890 doi (NATIONALLICENCE)springer-10.1007/BF00227890 Dillmann Wolfgang University of California, 92093, San Diego, La Jolla, CA, USA aut Regulation of expression of cardiac sarcoplasmic reticulum proteins under pathophysiological conditions [Elektronische Daten] [Wolfgang Dillmann] Congestive heart failure presents a significant medical problem and accumulating evidence indicates that slow relaxation during diastole maybe at least in part be medlated by decreased expression of the gene coding for the Ca2+ ATPase of the sarcoplasmic reticulum (SR). In order to determine if increased expression of the SR Ca2+ ATPase gene leads to alterations in calcium transients and in contractile behavior we constructed transgenic mice overexpressing the SERCA2 gene. Measuring dP/dtmax and dpPdtmin with a 2 French Milar catheter we found a significant Increase in systolic contraction and diastolic relaxation in transgene positive versus transgene negative mice. In addition we constructed adenoviruses overexpressing the gene coding for the Ca2+ ATPase of the sarcoplasmic reticulum. Infacting cardiac myocytes with the adenovirus expressing this transgene led to an accelerated calcium transient. Determining cell shortening and relengthening with a edge detection method indicated that increased expression of the SERCA2 transgene mediated by adenovirus Infection accelerated contractile parameters. In summary increased expression of the SERCA2 transgene leads to an enhancement of cardiac contrectile parameters under in vivo conditions in transgenic mice and in myocytes in cell culture using an adenovirus based approach to increase expression of the SERCAX gene. Kluwer Academic Publishers, 1996 congestive heart failure nationallicence cardiac relaxation nationallicence calcium ATPase of the sarcoplasmic reticulum nationallicence transgenic mice nationallicence adenoviruses nationallicence gene therapy nationallicence Molecular and Cellular Biochemistry Kluwer Academic Publishers 157/1-2(1996-04-01), 125-128 0300-8177 157:1-2<125 1996 157 11010 https://doi.org/10.1007/BF00227890 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227890 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Dillmann Wolfgang University of California, 92093, San Diego, La Jolla, CA, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 157/1-2(1996-04-01), 125-128 0300-8177 157:1-2<125 1996 157 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer