caa a22 4500 47712027X CHVBK 20180405111634.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00227884 doi (NATIONALLICENCE)springer-10.1007/BF00227884 Expression of cardiac muscle markers in rat myocyte cell lines [Elektronische Daten] [Gary Engelmann, Robert Worrell, Richard Duff, Patricia Grutkoski, Kenneth Chien, Richard Harvey] Recently developed rat heart myocyte cell lines have afforded us the opportunity to evaluate the expression of several transcription factors associated with early cardiac development. These factors include, but are not limited to, Nkx-2.5/Csx, MEF-2C and MLP (Muscle LIM Protein). These factors have been shown to be temporally expressed in pre-cardiac mesenchyme coincident with the earliest stages of heart development. Using the BWEM and CLEM myocyte cell lines as models of the embryonic, committed cardiomyocyte, we have evaluated the basal expression levels of these three genes over multiple passages. Both cell lines express these genes, with MEF-2C being the most abundant based on Northern blot hybridization analyses. Interestingly, as these cells increased their passage number, there was a corresponding increase in their basal expression levels. To evaluate potential ‘downstream' effectors of these genes, we examined the basal expression levels of two cardiac-specific genes cTNC and MLC-2v. Transcript levels for both of these contractile filament genes were elevated with passage, suggestive of a inductive process mediated by one or all of these three transcription factors. Promoter analysis of MLC-2v expression in the CLEM line shows that this increase is transcriptionally-mediated and the lines retain the necessary regulatory factors to maintain and control the transcription of this gene. Analysis of the dynamics of the regulatory role(s) that these three transcription factors play in cardiac development can now be evaluated in a homogeneous, cell culture system. Kluwer Academic Publishers, 1996 myocytes nationallicence myocyte cell lines nationallicence MLC-2v nationallicence MEF nationallicence MLP nationallicence TNC nationallicence MEF : muscle enhancer factor nationallicence MLP : muscle LIM protein nationallicence TNC : Troponin C nationallicence MLC : Myosin light chain nationallicence Engelmann Gary Department of Medicine and Molecular Biology Graduate Program, Cardiovascular Institute, Loyola University, 60153, Maywood, IL, USA aut Worrell Robert Department of Medicine and Molecular Biology Graduate Program, Cardiovascular Institute, Loyola University, 60153, Maywood, IL, USA aut Duff Richard Department of Medicine and Molecular Biology Graduate Program, Cardiovascular Institute, Loyola University, 60153, Maywood, IL, USA aut Grutkoski Patricia Department of Medicine and Molecular Biology Graduate Program, Cardiovascular Institute, Loyola University, 60153, Maywood, IL, USA aut Chien Kenneth Department of Medicine, Center for Molecular Genetics, University of California San Diego School of Medicine, San Diego, CA, USA aut Harvey Richard Walter and Eliza Hall Institute of Medical Research, 3050, Victoria, Australia aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 157/1-2(1996-04-01), 87-91 0300-8177 157:1-2<87 1996 157 11010 https://doi.org/10.1007/BF00227884 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00227884 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Engelmann Gary Department of Medicine and Molecular Biology Graduate Program, Cardiovascular Institute, Loyola University, 60153, Maywood, IL, USA aut NATIONALLICENCE 700 1- Worrell Robert Department of Medicine and Molecular Biology Graduate Program, Cardiovascular Institute, Loyola University, 60153, Maywood, IL, USA aut NATIONALLICENCE 700 1- Duff Richard Department of Medicine and Molecular Biology Graduate Program, Cardiovascular Institute, Loyola University, 60153, Maywood, IL, USA aut NATIONALLICENCE 700 1- Grutkoski Patricia Department of Medicine and Molecular Biology Graduate Program, Cardiovascular Institute, Loyola University, 60153, Maywood, IL, USA aut NATIONALLICENCE 700 1- Chien Kenneth Department of Medicine, Center for Molecular Genetics, University of California San Diego School of Medicine, San Diego, CA, USA aut NATIONALLICENCE 700 1- Harvey Richard Walter and Eliza Hall Institute of Medical Research, 3050, Victoria, Australia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 157/1-2(1996-04-01), 87-91 0300-8177 157:1-2<87 1996 157 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer