caa a22 4500 47712030X CHVBK 20180405111635.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00229745 doi (NATIONALLICENCE)springer-10.1007/BF00229745 Inhibition of rat parotid gland growth response induced by chronic isoproterenol following treatment with quinolone antibiotics [Elektronische Daten] [Barna Kelentey, Micah Kerr, Zeng Tao, Kamam Purushotham, Michael Humphreys-Beher, Tivadar Zelles] While antibiotics are broadly used in dental and medical therapy, little attention has been directed towards the potential toxic side effects of antibiotics on tissue regeneration. Here we examined the effect of a quinolone antibiotic, pefloxacin (Rhone Poulenc) on rat parotid gland responses to chronic isoproterenol treatment. Groups of rats received injections of isoproterenol to induce glandular growth, saline (controls), pefloxacin, or isoproterenol and pefloxacin in combination. Parotid gland weight decreased significantly after pefloxacin treatment for 7 days as well as inhibiting glandular enlargement provoked by isoproterenol. The same trend was observed for the rates of DNA synthesis, with the incorporation of [3H]-thymidine in isoproterenol/pefloxacin-treated rats reduced to 49% of isoproterenol treatment alone levels. Saline-treated animals were 42% of the rate of [3H]-thymidine incorporation into DNA observed in isoproterenol treated rats. While isoproterenol treatment increased steady-state mRNA levels for fos, jun, myc, src, c-erbB-2, ras and topo II, inclusion of pefloxacin with the isoproterenol regimen blocked these increases. Pefloxacin treatment by itself did not alter proto-oncogene mRNA levels in the parotid gland. Glandular amylase activity was decreased in the pefloxacin treated group, while the combination of isoproterenol with pefloxacin did not decrease glandular amylase levels to the extent of that observed with β-agonist treatment alone. In acute experiments, pefloxacin significantly decreased the volume of saliva secreted by the parotid gland. These results suggest that quinolone-based antibiotics disturb the secretory function of the parotid gland and can inhibit cell proliferation and regeneration. (Mol Cell Biochem 165: 55-63, 1996) Kluwer Academic Publishers, 1996 antibiotics nationallicence cell proliferation nationallicence salivary glands nationallicence secretion nationallicence Kelentey Barna Clinic of Stomatology, University of Debrecen, Debrecen, Hungary aut Kerr Micah Department of Oral Biology, University of Florida Health Science Center, P.O. Box 100424, 32610, Gainesville, FL, USA aut Tao Zeng Department of Oral Biology, University of Florida Health Science Center, P.O. Box 100424, 32610, Gainesville, FL, USA aut Purushotham Kamam Department of Oral Biology, University of Florida Health Science Center, P.O. Box 100424, 32610, Gainesville, FL, USA aut Humphreys-Beher Michael Department of Oral Biology, University of Florida Health Science Center, P.O. Box 100424, 32610, Gainesville, FL, USA aut Zelles Tivadar Department of Oral Biology, Sernmelweis University Medical School, Budapest, Hungary aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 165/1(1996-12-01), 55-63 0300-8177 165:1<55 1996 165 11010 https://doi.org/10.1007/BF00229745 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229745 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kelentey Barna Clinic of Stomatology, University of Debrecen, Debrecen, Hungary aut NATIONALLICENCE 700 1- Kerr Micah Department of Oral Biology, University of Florida Health Science Center, P.O. Box 100424, 32610, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Tao Zeng Department of Oral Biology, University of Florida Health Science Center, P.O. Box 100424, 32610, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Purushotham Kamam Department of Oral Biology, University of Florida Health Science Center, P.O. Box 100424, 32610, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Humphreys-Beher Michael Department of Oral Biology, University of Florida Health Science Center, P.O. Box 100424, 32610, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Zelles Tivadar Department of Oral Biology, Sernmelweis University Medical School, Budapest, Hungary aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 165/1(1996-12-01), 55-63 0300-8177 165:1<55 1996 165 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer