caa a22 4500 477120334 CHVBK 20180405111635.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00229740 doi (NATIONALLICENCE)springer-10.1007/BF00229740 Interaction of aldehydes with glyoxalase I and the status of several aldehyde metabolizing enzymes of Ehrlich ascites carcinoma cells [Elektronische Daten] [Swati Biswas, Shelly Bhattacharjee, Manju Ray, Subhankar Ray] The possible effect of several physiologically important aldehydes has been tested on partially purified glyoxalase I of Ehrlich ascites carcinoma (EAC) cells. The results indicate that D, and L-lactaldehyde are strong non-competitive inhibitors of glyoxalase I and the effect with the D-isomer is more pronounced, whereas both D,L-glyceraldehyde and acetaldehyde are moderately inhibitory and the nature of inhibition is strictly competitive. Moreover, D,L-glyceraldehyde strongly inhibits the utilization of methylglyoxal by intact EAC cells. A search for the presence of several aldehyde metabolizing enzymes in EAC cells indicates that non-specific aldehyde reductase, methylglyoxal reductase, aldehyde dehydrogenase and alcohol dehydrogenase are apparently absent in this rapidly growing, highly de-differentiated malignant cell. Kluwer Academic Publishers, 1996 methylglyoxal nationallicence glyoxalase I nationallicence aldehydes nationallicence cancer nationallicence Biswas Swati Department of Biological Chemistry, Indian Association for the Cultivation of Science, 700 032, Calcutta aut Bhattacharjee Shelly Department of Biological Chemistry, Indian Association for the Cultivation of Science, 700 032, Calcutta aut Ray Manju Department of Biological Chemistry, Indian Association for the Cultivation of Science, 700 032, Calcutta aut Ray Subhankar Department of Biochemistry, University College of Science, University of Calcutta, 700 019, Calcutta, India aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 165/1(1996-12-01), 9-16 0300-8177 165:1<9 1996 165 11010 https://doi.org/10.1007/BF00229740 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229740 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Biswas Swati Department of Biological Chemistry, Indian Association for the Cultivation of Science, 700 032, Calcutta aut NATIONALLICENCE 700 1- Bhattacharjee Shelly Department of Biological Chemistry, Indian Association for the Cultivation of Science, 700 032, Calcutta aut NATIONALLICENCE 700 1- Ray Manju Department of Biological Chemistry, Indian Association for the Cultivation of Science, 700 032, Calcutta aut NATIONALLICENCE 700 1- Ray Subhankar Department of Biochemistry, University College of Science, University of Calcutta, 700 019, Calcutta, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 165/1(1996-12-01), 9-16 0300-8177 165:1<9 1996 165 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer