caa a22 4500 477120342 CHVBK 20180405111635.0 cr unu---uuuuu 170330e19961201xx s 000 0 eng 10.1007/BF00229742 doi (NATIONALLICENCE)springer-10.1007/BF00229742 Synergistic effect of BMP-2 and ascorbate on the phenotypic expression of osteoblastic MC3T3-E1 cells [Elektronische Daten] [Yasuyoshi Torii, Kiyotaka Hitomi, Norihiro Tsukagoshi] Of TGF-β superfamily proteins, BMP-2 enhanced alkaline phosphatase (ALP) activity in cultured osteoblastic cells, MC3T3-E1, to the same level promoted by ascorbate, whereas TGF-βs (β1, β2, β3) reduced ALP activity and altered cell morphology under the same conditions. Activin appeared to have no distinct effect on ALP synthesis. Ascorbate dependent increase in ALP synthesis was markedly stimulated in the presence of BMP-2. The synergistic effect of ascorbate on ALP synthesis was replaced by type I collagen coated on the culture dish. However, BMP-2 appeared not to bind to type I collagen. These findings indicate that BMP-2 acts directly on osteoblastic cells through its receptors and collagenous matrix can neither recruit BMP-2 nor modulate directly the action of BMP-2 in the pericellular area. Treatment of cells grown in ascorbate media with TGF-βs decreased rapidly the cellular ALP activity indicating that TGF-βs direct cells to the differentiated stage. Kluwer Academic Publishers, 1996 MC3T3-E1 nationallicence osteoblast nationallicence ascorbate nationallicence TGF-β nationallicence BMP-2 nationallicence alkaline phosphatase nationallicence Torii Yasuyoshi Department of Applied Biological Sciences, Faculty of Agriculture, Nagoya University, 464-01, Nagoya, Japan aut Hitomi Kiyotaka Department of Applied Biological Sciences, Faculty of Agriculture, Nagoya University, 464-01, Nagoya, Japan aut Tsukagoshi Norihiro Department of Applied Biological Sciences, Faculty of Agriculture, Nagoya University, 464-01, Nagoya, Japan aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 165/1(1996-12-01), 25-29 0300-8177 165:1<25 1996 165 11010 https://doi.org/10.1007/BF00229742 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00229742 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Torii Yasuyoshi Department of Applied Biological Sciences, Faculty of Agriculture, Nagoya University, 464-01, Nagoya, Japan aut NATIONALLICENCE 700 1- Hitomi Kiyotaka Department of Applied Biological Sciences, Faculty of Agriculture, Nagoya University, 464-01, Nagoya, Japan aut NATIONALLICENCE 700 1- Tsukagoshi Norihiro Department of Applied Biological Sciences, Faculty of Agriculture, Nagoya University, 464-01, Nagoya, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www.wkap.nl 165/1(1996-12-01), 25-29 0300-8177 165:1<25 1996 165 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer